Species-specific polymerase chain reaction amplification of camel (Camelus) DNA extracts

A sensitive polymerase chain reaction (PCR) method based on amplification of a specific DNA fragment was established for the identification of camel (Camelus) materials. The species-specific primer pair L183/H372 was designed based on the nucleotide sequence of the mitochondrial cytochrome b gene, a...

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Bibliographic Details
Published in:Journal of AOAC International 2005-09, Vol.88 (5), p.1394-1398
Main Authors: Chen, Y, Wu, Y.J, Xu, B.L, Wan, J, Qian, Z.M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
camel meat
Camels
Camelus - classification
Camelus - genetics
Cattle
cytochrome b
Cytochromes b - genetics
DNA
DNA - classification
DNA Primers
Dogs
Food
Food Analysis - methods
Food industries
Fundamental and applied biological sciences. Psychology
Genetic aspects
Identification and classification
meat and bone meal
Methods
molecular sequence data
Nucleic Acid Amplification Techniques - methods
nucleotide sequences
Polymerase chain reaction
Polymerase Chain Reaction - methods
product authenticity
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Sequence Alignment
Sequence Analysis, DNA
Species Specificity
Testing
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Summary:A sensitive polymerase chain reaction (PCR) method based on amplification of a specific DNA fragment was established for the identification of camel (Camelus) materials. The species-specific primer pair L183/H372 was designed based on the nucleotide sequence of the mitochondrial cytochrome b gene, and its specificity was confirmed by amplification of 3 camel (domestic double-humped camel, wild double-humped camel, wild one-humped camel) samples and 11 non-Camelus animal (sheep, goat, pig, chicken, cattle, fish, dog, horse, donkey, deer, and rabbit) materials. An expected 208 base pair fragment was amplified from camel materials; no cross-reactive or additional fragments were generated from other animal materials. Taq I restriction endonuclease digestion of the unpurified PCR product can be used routinely to confirm the camel origin of the amplified sequence.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/88.5.1394