Treatment with 1, 25-Dihydroxyvitamin D3 Preserves Glomerular Slit Diaphragm-Associated Protein Expression in Experimental Glomerulonephritis

In this study, we investigated the effect of 1,25(OH)2D3 on proteinuria and on the alteration of slit diaphragm-associated proteins induced by anti-Thy 1.1 in Wistar rats. Four groups of animals were studied: group I, anti-Thy 1.1 treated rats; group II, anti-Thy1.1 treated group that at day 2, afte...

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Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2005-10, Vol.18 (4), p.779-790
Main Authors: Migliori, M., Giovannini, L., Panichi, V., Filippi, C., Taccola, D., Origlia, N., Mannari, C., Camussi, G.
Format: Article
Language:English
Subjects:
Animals
Calcitriol - therapeutic use
Desmin - biosynthesis
Female
Fluorescent Antibody Technique
Glomerulonephritis, Membranoproliferative - drug therapy
Glomerulonephritis, Membranoproliferative - metabolism
Glomerulonephritis, Membranoproliferative - pathology
Kidney Glomerulus - drug effects
Kidney Glomerulus - metabolism
Kidney Glomerulus - pathology
Membrane Proteins - biosynthesis
Phosphoproteins - biosynthesis
Polymers - metabolism
Proteinuria - metabolism
Rats
Rats, Wistar
Tissue Fixation
Vitamins - therapeutic use
Zonula Occludens-1 Protein
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Summary:In this study, we investigated the effect of 1,25(OH)2D3 on proteinuria and on the alteration of slit diaphragm-associated proteins induced by anti-Thy 1.1 in Wistar rats. Four groups of animals were studied: group I, anti-Thy 1.1 treated rats; group II, anti-Thy1.1 treated group that at day 2, after the onset of overt proteinuria, started the treatment with 1,25(OH)2D3; group III, normal control rats injected with vehicle alone; group IV, rats that received only 1,25(OH)2D3. At day 2, in group I and II, before the administration of 1,25(OH)2D3, protein excretion was significantly increased when compared to controls. Overt proteinuria was maintained until day 14 in group I whereas in group II protein excretion was significantly reduced from day 3 to day 14. Moreover, treatment with 1,25(OH)2D3 abrogated podocytes injury, detected as desmin expression and loss of nephrin and zonula occludens-1 (ZO-1), two slit diaphragm-associated proteins, and glomerular polyanion staining, that were observed in group I. In conclusion, these results suggest that 1,25(OH)2D3 administrated with a therapeutic regiment may revert proteinuria, counteracting glomerular podocyte injury.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463200501800422