Evaluation of Liver Damage After Application of TVE in the Rat Model

• Published in: Transplantation proceedings 2005-12, Vol.37 (10), p.4550-4552
• Main Authors: Darilmaz, G., Topaloglu, S., Topaloglu, E., Ozel, H., Saygun, O., Avsar, F.M., Sokmensuer, C., Ucar, G., Sahin, M., Hengirmen, S.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Catalase - metabolism; Disease Models, Animal; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glutathione - metabolism; Glutathione Transferase - metabolism; Ischemia - pathology; Ischemia - physiopathology; Liver - blood supply; Liver - metabolism; Liver - pathology; Liver Circulation - physiology; Male; Malondialdehyde - metabolism; Medical sciences; Oxidative Stress - physiology; Rats; Rats, Wistar; Reperfusion Injury - pathology; Reperfusion Injury - physiopathology; Superoxide Dismutase - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2005.11.002

The aim of this study was to investigate the effects of total vascular exclusion (TVE) on the liver during the early period of reperfusion. Forty Wistar-Albino rats were divided into four groups. Portal pedicle clamping (groups 1 and 2) or TVE (groups 3 and 4) were applied for 10 minutes. Samples were collected at the time of clamp release (groups 1 and 3) and at 30 minutes of reperfusion (groups 2 and 4). We examined oxidative injury to and histopathology of the liver. Oxidative stress was more prominent with TVE application. Significant alterations were shown in hepatic superoxide dismutase, catalase, glutathione, and glutathione S-transferase levels. The levels of malondialdehyde and myeloperoxidase were not altered significantly. Inflow-outflow occlusion of the liver causes more oxidative stress compared with inflow occlusion.