Histological Study of Macroencapsulation of Pancreatic Islet Cells After Transplantation Into the Bloodstream

Experimental dogs with alloxan-induced diabetes mellitus ( n = 22) were transplanted into the abdominal aorta lumen with macroencapsulated xenogenic rabbit islet cells without immunosuppressive therapy. The investigation showed normalization of the blood glucose level and disappearance of diabetes m...

Full description

Saved in:
Bibliographic Details
Published in:Transplantation proceedings 2005-12, Vol.37 (10), p.4446-4448
Main Authors: Prochorov, A.V., Roudenok, V.V., Goranov, V.A.
Format: Article
Language:English
Subjects:
Animals
Aorta, Abdominal
Biological and medical sciences
Blood Glucose - metabolism
Capsules
Diabetes Mellitus, Experimental - immunology
Diabetes Mellitus, Experimental - surgery
Diabetes. Impaired glucose tolerance
Disease Models, Animal
Dogs
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Injections
Insulin - metabolism
Insulin Secretion
Islets of Langerhans Transplantation - immunology
Islets of Langerhans Transplantation - methods
Islets of Langerhans Transplantation - pathology
Medical sciences
Rabbits
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
Transplantation, Heterologous
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Experimental dogs with alloxan-induced diabetes mellitus ( n = 22) were transplanted into the abdominal aorta lumen with macroencapsulated xenogenic rabbit islet cells without immunosuppressive therapy. The investigation showed normalization of the blood glucose level and disappearance of diabetes mellitus symptoms. Morphological and immunohistochemical investigations 13 months later demonstrated preservation and functional activity of up to 60% of the transplanted islet cells. Meanwhile by month 6 a structure similar to the Islet of Langerhans had formed in the capsule. The majority of the mass of cells was insulin-positive by immunohistochemical investigation. The bloodstream appears to be an immune-privileged zone, where no graft rejection develops and long-term preservation-function of xenogenic islet cells may be possible. Macroencapsulated islets transplanted into the vascular lumen may form an endocrine apparatus of a bioartificial pancreas.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2005.10.052