Plectin rodless isoform expression and its detection in mouse brain

The widely expressed cytolinker protein plectin shows extensive isoform diversity both at the N-terminus and in the central part of the molecule. Judged on mRNA data, plectin variants lacking the central rod domain are expressed at a approximately 20-fold lower level than full-length proteins and th...

Full description

Saved in:
Bibliographic Details
Published in:Cellular and molecular neurobiology 2005-11, Vol.25 (7), p.1141
Main Authors: Fuchs, Peter, Spazierer, Daniel, Wiche, Gerhard
Format: Article
Language:English
Subjects:
Alternative Splicing - genetics
Animals
Biological Assay
Brain - metabolism
DNA, Complementary - genetics
Exons - genetics
Gene Expression Regulation, Enzymologic - genetics
Glioma - genetics
Mice
Plectin - analysis
Plectin - chemistry
Plectin - genetics
Plectin - metabolism
Protein Isoforms - analysis
Protein Isoforms - chemistry
Protein Isoforms - genetics
Protein Isoforms - metabolism
Rats
Ribonucleases - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The widely expressed cytolinker protein plectin shows extensive isoform diversity both at the N-terminus and in the central part of the molecule. Judged on mRNA data, plectin variants lacking the central rod domain are expressed at a approximately 20-fold lower level than full-length proteins and their detection on the protein level can be difficult. Here we present data on the expression of plectin rodless isoforms in mouse brain and in rat glioma C6 cells on RNA and protein levels. Our data indicate that among the rodless variants expressed in neuronal tissues, those starting with exon 1c (plectin 1c) seem to be the most prominent ones. In addition, we show that similar to other monoclonal antibodies reported in the literature, the widely used mAb 7A8 recognizes an epitope within plectin's rod domain and therefore is unsuited to detect rodless variants of plectin.
ISSN:0272-4340
DOI:10.1007/s10571-005-7826-1