Prevalence of four virulence genes in Campylobacter jejuni determined by PCR and sequence analysis

The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence. The virulence genes were detected by...

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Bibliographic Details
Published in:Molecular diagnosis 2005, Vol.9 (4), p.211-215
Main Authors: Kordinas, Vasilios, Nicolaou, Chryssoula, Ioannidis, Anastassios, Papavasileiou, Eleni, John Legakis, Nicolaos, Chatzipanagiotou, Stylianos
Format: Article
Language:English
Subjects:
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
Campylobacter jejuni - pathogenicity
Child
Feces - microbiology
Gastroenteritis - microbiology
Genes, Bacterial
Guillain-Barre Syndrome - microbiology
Humans
Miller Fisher Syndrome - microbiology
Polymerase Chain Reaction
Sequence Analysis
Virulence
Virulence Factors - genetics
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Summary:The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence. The virulence genes were detected by PCR and the amplified products were submitted for sequence analysis. The gene with the highest prevalence was racR (87.08%). virB was present in only 1.69% of the C. jejuni strains, and wlaN and cgtB were detected in 16.01% and 24.44%, respectively. Five strains associated with Guillain-Barré syndrome and Miller-Fischer syndrome out of the total of 356 (1.40%) were positive for cgtB. Our findings suggest that racR may encode factors necessary for bacterial pathogenicity in humans, while the roles of the other three genes remain ambiguous.
ISSN:1084-8592