Molecular Characterization of Avian Infectious Bronchitis Virus Strains Isolated in Colombia During 2003

Sixteen infectious bronchitis virus (IBV) isolates were recovered from broilers and layers from five geographic poultry regions in Colombia. The viruses were isolated from tracheas, lungs, and cecal tonsils of birds, previously vaccinated with the Massachusetts strain, that were showing respiratory...

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Bibliographic Details
Published in:Avian diseases 2005-12, Vol.49 (4), p.494-499
Main Authors: Alvarado, I. R, Villegas, P, Mossos, N, Jackwood, M. W
Format: Article
Language:English
Subjects:
amino acid sequences
Amino acids
Animals
Base Sequence
broiler chickens
Chickens
Chickens - virology
Colombia
Coronavirus Infections - veterinary
Coronavirus Infections - virology
cycle sequencing
Female
genes
Genes, Viral
genetic variation
Genotype
Genotypes
Geographic regions
geographical variation
Glycoproteins
HVR = hypervariable region
IB = infectious bronchitis
IBV
IBV = infectious bronchitis virus
infectious bronchitis
Infectious bronchitis virus
Infectious bronchitis virus - classification
Infectious bronchitis virus - genetics
Infectious bronchitis virus - isolation & purification
laying hens
lungs
molecular sequence data
Phylogeny
Polymerase chain reaction
Poultry Diseases - virology
Regular s
Reverse transcriptase polymerase chain reaction
RNA, Viral - genetics
RNA, Viral - isolation & purification
RT-PCR
RT-PCR = reverse transcriptase–polymerase chain reaction
S glycoprotein = spike glycoprotein
S1 gene
SPF = specific-pathogen-free
strain differences
strains
tonsils
trachea (vertebrates)
Vaccination
variants
viral proteins
Viruses
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Description
Summary:Sixteen infectious bronchitis virus (IBV) isolates were recovered from broilers and layers from five geographic poultry regions in Colombia. The viruses were isolated from tracheas, lungs, and cecal tonsils of birds, previously vaccinated with the Massachusetts strain, that were showing respiratory signs. Further analysis of the IBV isolates was achieved by phylogenetic analysis comparing their deduced amino acid sequences in the hypervariable region 1 of the S1 gene with reference strains. Four unique genotype clusters containing isolates with indigenous genotypes were observed. One isolate was found to be the Connecticut genotype and three isolates were found to be the Massachusetts genotype.
ISSN:0005-2086
1938-4351
DOI:10.1637/7202-050304R.1