Immunohistochemical staining of the astrocytic expression of glial fibrillary acidic protein and vimentin in the central nervous system of dogs with canine distemper

• Published in: Arquivos de neuro-psiquiatria 2007-12, Vol.65 (4A), p.1070-1077
• Main Authors: Orsini, Heloísa, Bondan, Eduardo Fernandes, Sanchez, Melissa, Lallo, Maria Anete, Maiorka, Paulo César, Dagli, Maria Lúcia Zaidan, Graça, Dominguita Luthers
• Format: Article
• Language: Portuguese
• Subjects: Animals; Astrocytes - metabolism; Astrocytes - pathology; Astrocytes - virology; Canine distemper virus; Demyelinating Diseases - pathology; Demyelinating Diseases - virology; Distemper - metabolism; Distemper - pathology; Dogs; Glial Fibrillary Acidic Protein - metabolism; Immunohistochemistry - veterinary; Vimentin - metabolism
• ISSN: 0004-282X
• DOI: 10.1590/S0004-282X2007000600032

Considering that many aspects involved in the pathogenesis of the central nervous system (CNS) demyelinating diseases are still poorly understood and that astrocytes seem to mediate such processes, this study analyzed the participation of astrocytes in the demyelinating processes of CNS by using immunohistochemical staining of two astrocytic proteins--glial fibrillary acidic protein (GFAP) and vimentin (VIM)--comparing samples of cerebellum and brainstem from eight dogs with canine distemper and from two healthy dogs, from different breeds and ages varying from 1 to 4 years old. Histological sections were submitted to the avidin-biotin-peroxidase indirect method of immunohistochemical staining (ABC) and the astrocytic reactivity, observed in light microscopy, was quantified in a computer system for image analysis. It was possible to notice, on most of the sections from sick animals, degenerative lesions that indicate demyelination. The immunostaining for GFAP and VIM was more intense on animals with canine distemper, specially around the ventricules and near degenerated sites. There was no significant difference between the immunostaining (GFAP and VIM) of animals with canine distemper with and without inflammatory infiltrate of the cerebellar white matter. The increased immunoreactivity of astrocytes for GFAP and the VIM reexpression in injured areas indicate the astrocytic involvement on nervous tissue response to the demyelinating lesions induced by the canine distemper virus (CDV) in the CNS.