Examining the microanatomy of various fetal body parts using the orcein-picroindigocarmine staining method

It is difficult to distinguish muscle, vessel, nerve, fascia, tendon and many other structures in fetal sections. The aim of the study reported here was to research the practicability of orcein-picroindigocarmine staining for distinguishing anatomic structures in histological sections containing com...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2007, Vol.82 (6), p.319-323
Main Authors: Demirta, K, Gülekon, N, Peker, T, Ömero lu, S, Anil, A, Turgut, Hb
Format: Article
Language:English
Subjects:
Carmine - analogs & derivatives
Coloring Agents
Fetus - cytology
Fetuses
Humans
Image Enhancement - methods
orcein-picroindigocarmine stain
Oxazines
sectional anatomy
Staining and Labeling - methods
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Summary:It is difficult to distinguish muscle, vessel, nerve, fascia, tendon and many other structures in fetal sections. The aim of the study reported here was to research the practicability of orcein-picroindigocarmine staining for distinguishing anatomic structures in histological sections containing complex structures. Histological serial sections of hand, foot, head and neck of four 18- to 20-week old fetuses were used. Bone matrix was stained bright blue, collagen fibers tones of green to blue, elastic fibers brown, cartilage matrix tones of pink, and chondroblasts blue. Muscles and erythrocytes were stained yellow to green, epidermis reddish brown, skin appendices including hair and nail stained light yellow to green.
ISSN:1052-0295
1473-7760
DOI:10.1080/10520290701822477