X and Y chromosome inheritance and mixtures of rDNA intergenic spacer regions in Anopheles gambiae

We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M and S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated with M...

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Bibliographic Details
Published in:Insect molecular biology 2007-12, Vol.16 (6), p.735-741
Main Authors: Wilkins, E.E, Howell, P.I, Benedict, M.Q
Format: Article
Language:English
Subjects:
Animals
Anopheles - genetics
Base Sequence
chromosomal form
Crosses, Genetic
DNA, Ribosomal Spacer - genetics
Female
Genetic Linkage
Genotype
IGS
Insect Vectors - genetics
Male
Mopti
Polymorphism, Single Nucleotide
rDNA
recombination
Savanna
savannas
Sequence Homology, Nucleic Acid
sex determination
X Chromosome - genetics
Y Chromosome - genetics
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Summary:We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M and S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated with M and S. Standard M and S stocks demonstrated X-chromosome-only inheritance of the rDNA form, but the ASEMBO1 males showed X and Y chromosome linkage of mixed rDNA. The metaphase Y chromosomes of ASEMBO1 contained a significantly larger amount of DNA relative to the X than a standard S stock. Analysis of wild A. gambiae males from the putative location of origin of the ASEMBO1 stock did not detect the same pattern of polymorphism observed in the laboratory stock but did detect heterogeneous arrays including some missing a diagnostic Hha I restriction site. These results demonstrate that M and S IGS types can occur within the rDNA arrays of a chromatid in laboratory A. gambiae stocks, and some A. gambiae s.s. have rDNA on the Y chromosome.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2007.00769.x