Tracking the Elusive Lymphocyte: Methods of Detection during Adoptive Immunotherapy

Adoptive immunotherapy is an attractive cancer treatment modality due to its capacity to target primary and metastatic lesions with large numbers of tumor-reactive, cytotoxic lymphocytes. The inability of fully armed lymphocytes to traffic into sites of tumor has been proposed as a causal factor for...

Full description

Saved in:
Bibliographic Details
Published in:Immunological investigations 2007, Vol.36 (5-6), p.807-827
Main Authors: Skitzki, Joseph J., Muhitch, Jason B., Evans, Sharon S.
Format: Article
Language:English
Subjects:
adoptive immunotherapy
Animals
cancer
CD45
CFSE
Fluorescent Dyes - pharmacokinetics
Fluorescent Dyes - pharmacology
Immunotherapy, Adoptive - methods
Iodine Radioisotopes
lymphocyte trafficking
Lymphocytes - immunology
Lymphocytes, Tumor-Infiltrating - drug effects
Lymphocytes, Tumor-Infiltrating - metabolism
Lymphocytes, Tumor-Infiltrating - physiology
Mice
Mice, Congenic
Models, Biological
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - therapy
Organic Chemicals
Tissue Distribution
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adoptive immunotherapy is an attractive cancer treatment modality due to its capacity to target primary and metastatic lesions with large numbers of tumor-reactive, cytotoxic lymphocytes. The inability of fully armed lymphocytes to traffic into sites of tumor has been proposed as a causal factor for the minimal success observed clinically with this type of immunotherapy. The study of lymphocyte trafficking during adoptive immunotherapy has been limited, despite the existence of a variety of tracking methods. In murine models that simulate adoptive immunotherapy, the use of congenic mice and cell tracking dyes can be used to elucidate lymphocyte trafficking behavior. The continued development of novel technologies will further contribute to this expanding area of research.
ISSN:0882-0139
1532-4311
DOI:10.1080/08820130701712867