Antifibrotic Phenanthrenes of Dendrobium nobile Stems

• Published in: Journal of natural products (Washington, D.C.) D.C.), 2007-12, Vol.70 (12), p.1925-1929
• Main Authors: Yang, Hyekyung, Sung, Sang Hyun, Kim, Young Choong
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; cell proliferation; Cell Proliferation - drug effects; chemical structure; cultured cells; Dendrobium; Dendrobium - chemistry; Dendrobium nobile; dihydrophenanthrene; fibrosis; General pharmacology; hepatocytes; Hepatocytes - drug effects; human health; Korea; liver cirrhosis; Medical sciences; medicinal plants; medicinal properties; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; phenanthrene; Phenanthrenes - chemistry; Phenanthrenes - isolation & purification; Phenanthrenes - pharmacology; Plant Stems - chemistry; Plants, Medicinal - chemistry; Rats; spectral analysis; trimethoxyphenanthrene
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np070423f

Two new phenanthrenes (1 and 6) and four new dihydrophenanthrenes (2–5) were isolated from a methanolic extract of Dendrobium nobile stems, along with 13 known phenanthrenes and dihydrophenanthrenes (7–19). By spectroscopic analysis, the structures of compounds 1–6 were determined as 2,8-dihydroxy-3,4,7-trimethoxyphenanthrene (1), 3-hydroxy-2,4,7-trimethoxy-9,10-dihydrophenanthrene (2), 2,8-dihydroxy-3,4,7-trimethoxy-9,10-dihydrophenanthrene (3), 2-hydroxy-4,7-dimethoxy-9,10-dihydrophenanthrene (4), 2,2′-dihydroxy-3,3′,4,4′,7,7′-hexamethoxy-9,9′,10,10′-tetrahydro-1,1′-biphenanthrene (5), and 2,3,5-trihydroxy-4,9-dimethoxyphenanthrene (6), respectively. Antifibrotic activity of compounds 1–19 was evaluated employing HSC-T6, an immortalized rat hepatic stellate cell line, as an in vitro assay system by assessing cell proliferation.