Effect of Aging on the Expression of Adrenomedullin and Its Receptor Component Proteins in the Male Reproductive System of the Rat

This study investigated the levels of adrenomedullin (AM) and the gene expression of AM, calcitonin receptor-like receptor (CRLR), receptor activity-modifying proteins (RAMPs), and receptor-coupling protein (RCP) in the testis, ventral prostate, seminal vesicle, and epididymis in rats aged 3, 12, an...

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Bibliographic Details
Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2007-12, Vol.62 (12), p.1346-1351
Main Authors: Li, Yuk-Yin, O, Wai-Sum, Tang, Fai
Format: Article
Language:English
Subjects:
Adrenomedullin - analysis
Adrenomedullin - genetics
Adrenomedullin - physiology
Age
Aging - metabolism
Amino acids
Animals
Binding sites
Calcitonin Receptor-Like Protein
DNA polymerase
Endocrine system
Genitalia, Male - chemistry
Genitalia, Male - metabolism
Gerontology
Intracellular Signaling Peptides and Proteins - genetics
Laboratory animals
Male
Membrane Proteins - genetics
Molecular weight
Plasma
Proteins
Rats
Rats, Sprague-Dawley
Receptor Activity-Modifying Proteins
Receptors, Calcitonin - genetics
Receptors, Calcitonin Gene-Related Peptide - genetics
Reproductive system
RNA, Messenger - analysis
Rodents
Signal transduction
Testosterone
Testosterone - blood
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Summary:This study investigated the levels of adrenomedullin (AM) and the gene expression of AM, calcitonin receptor-like receptor (CRLR), receptor activity-modifying proteins (RAMPs), and receptor-coupling protein (RCP) in the testis, ventral prostate, seminal vesicle, and epididymis in rats aged 3, 12, and 20 months by radioimmunoassay and semiquantitative reverse transcription–polymerase chain reaction (RT–PCR). The results indicate an age-related increase in AM and its messenger RNA (mRNA) levels in the testis and decrease in the sex accessory glands. The gene expression of CRLR and RCP decreased only in the sex accessory glands. The changes in the gene expression of RAMPs suggest that the major increase was in CGRP receptors in the testis, whereas the major deceases in the ventral prostate and the seminal vesicles might be CGRP and AM receptors, respectively. The decreases in these receptors were similar in the epididymis. The results suggest possible roles of AM in the male reproductive system during aging.
ISSN:1079-5006
1758-535X
DOI:10.1093/gerona/62.12.1346