Calcium binding protein calcyphosine in dog central astrocytes and ependymal cells and in peripheral neurons

Calcyphosine is a calcium binding protein discovered in the dog thyroid in 1979. Calcyphosine mRNA and immunoreactivity were detected using Western and Northern blotting in the cerebral cortex, cerebral white matter and cerebellum. Using immunohistochemistry and in situ hybridization, both are prese...

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Bibliographic Details
Published in:Journal of chemical neuroanatomy 1998-10, Vol.15 (4), p.239-250
Main Authors: Halleux, P, Schurmans, Stephane, Schiffmann, Serge N, Lecocq, Raymond, Conreur, Jean-Louis, Dumont, Jacques, Vanderhaeghen, Jean-Jacques
Format: Article
Language:English
Subjects:
Adrenal medulla
Animals
Astrocytes
Astrocytes - metabolism
Blotting, Northern
Blotting, Western
Brain - cytology
Brain Chemistry - physiology
Calcium-Binding Proteins - metabolism
Choroid plexuses
Dogs
Dorsal root ganglia
Enteric nervous system
Ependyma - cytology
Ependyma - metabolism
Ependymal cells
Immunohistochemistry
Neurons - metabolism
Peripheral Nervous System - cytology
Peripheral Nervous System - metabolism
RNA, Messenger - biosynthesis
Spinal Cord - cytology
Spinal Cord - metabolism
Sympathetic ganglia
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Summary:Calcyphosine is a calcium binding protein discovered in the dog thyroid in 1979. Calcyphosine mRNA and immunoreactivity were detected using Western and Northern blotting in the cerebral cortex, cerebral white matter and cerebellum. Using immunohistochemistry and in situ hybridization, both are present in ependymal cells, choroid plexus cells and several types of astrocytes of the subependymal cerebral layer, the cerebellar Bergmann layer, the retinal ganglion cell layer, the optic nerve and the posterior pituitary. Both are also present in neurons of nasal olfactory mucosa, enteric Auerbach and Meissner plexuses, orthosympathic and spinal cord ganglia as well as in endocrine cells of neural crest origin in the adrenal medulla. Calcyphosine immunoreactive astrocytes were also present mainly in hemispheric cerebral gray and white matter, hemispheric subcortical structures, brain stem and spinal cord. These results show that calcyphosine is a characteristic calcium binding protein of astrocytes and ependymal cells in the central nervous system and of neurons in the peripheral nervous system. This is of interest in view of the importance of calcium regulation in these cells, and since calcyphosine a calcium binding protein phosphorylated by cAMP dependent process, may be an intermediate between cAMP and inositol phosphate cascades.
ISSN:0891-0618
1873-6300
DOI:10.1016/S0891-0618(98)00049-0