Evaluation of anesthetic effects on parameters for the in situ rat brain perfusion technique

Studies of drug distribution to brain should be controlled for the experimental method used. Numerous methods have been employed to ascertain brain distribution and many of these approaches use anesthetic agents. The in situ rat brain perfusion method is one of the most sensitive and widely used met...

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Bibliographic Details
Published in:Neuroscience letters 1998-12, Vol.257 (3), p.139-142
Main Authors: Rousselle, Christophe H, Lefauconnier, Jeanne-Marie, Allen, David D
Format: Article
Language:English
Subjects:
Anesthetics
Anesthetics - pharmacology
Anesthetics. Neuromuscular blocking agents
Animals
Biological and medical sciences
Blood-Brain Barrier - drug effects
Blood–brain barrier
Brain - blood supply
Brain - drug effects
Brain vascular volume
Cerebral fluid flow
Cerebrovascular Circulation - drug effects
Ketamine
Male
Medical sciences
Neuropharmacology
Pentobarbital
Perfusion - methods
Permeability
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
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Summary:Studies of drug distribution to brain should be controlled for the experimental method used. Numerous methods have been employed to ascertain brain distribution and many of these approaches use anesthetic agents. The in situ rat brain perfusion method is one of the most sensitive and widely used methods for evaluating brain distribution profiles. There has been no evaluation of the effects of anesthetic agents on parameters associated with this method (i.e. cerebral perfusion fluid flow, brain vascular volume and blood–brain barrier permeability). We evaluated the effects of the anesthetic agents pentobarbital and ketamine combinations on these baseline parameters. The results suggest that the anesthetic agent has no effect on these parameters and anesthetic selection is open to the choice of the investigator when using the perfusion method.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(98)00827-1