Recent studies of T7 RNA polymerase mechanism

Bacteriophage T7 RNA polymerase (T7 RNAP) is known to be one of the simplest enzymes catalyzing RNA synthesis. In contrast to most RNA polymerases known, this enzyme consists of one subunit and is able to carry out transcription in the absence of additional protein factors. Owing to its molecular pr...

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Bibliographic Details
Published in:FEBS Letters 1998-12, Vol.440 (3), p.264-267
Main Authors: Kochetkov, S.N, Rusakova, E.E, Tunitskaya, V.L
Format: Article
Language:English
Subjects:
Bacteriophage T7
Base Sequence
DNA, Viral - metabolism
DNA-Directed RNA Polymerases - chemistry
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
DNAP, DNA polymerase
Elongation
Enzyme-promoter interaction
Initiation
Mechanism
Molecular Sequence Data
Mutagenesis
Promoter Regions, Genetic
Ribonucleotides - metabolism
RNAP, RNA polymerase
Sequence Homology, Nucleic Acid
T7 RNA polymerase
Transcription
Transcription, Genetic
Viral Proteins
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Summary:Bacteriophage T7 RNA polymerase (T7 RNAP) is known to be one of the simplest enzymes catalyzing RNA synthesis. In contrast to most RNA polymerases known, this enzyme consists of one subunit and is able to carry out transcription in the absence of additional protein factors. Owing to its molecular properties, the enzyme is widely used for synthesis of specific transcripts, as well as being a suitable model for studying the mechanisms of transcription. In this minireview the recent data on the structure and mechanism of T7 RNAP, including enzyme-promoter interactions, principal stages of transcription, and the results of functional studies are discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)01484-7