Genetic relationships among Japanese native breeds of chicken based on microsatellite DNA polymorphisms

Genetic relationships among Japanese native breeds of chickens were studied on the basis of microsatellite DNA polymorphisms. DNA samples from 10 Japanese native breeds (Iwate-Jidori, Aizu-Jidori, Sadohige-Jidori, Siba-Tori, Onaga-Dori, Echigonankin, Hinai, Kinpa, Koeyoshi, and Tomaru) and one impor...

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Bibliographic Details
Published in:The Journal of heredity 1998-11, Vol.89 (6), p.543-546
Main Authors: Takahashi, H, Nirasawa, K, Nagamine, Y, Tsudzuki, M, Yamamoto, Y
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Birds
Breeding of animals
CHICKENS
Chickens - genetics
Deoxyribonucleic acid
DNA
DNA Primers
DNA, Satellite - genetics
Genetics
JAPAN
JAPON
Microsatellite Repeats
POLLO
Polymorphism, Genetic
POULET
Poultry
Species Specificity
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Summary:Genetic relationships among Japanese native breeds of chickens were studied on the basis of microsatellite DNA polymorphisms. DNA samples from 10 Japanese native breeds (Iwate-Jidori, Aizu-Jidori, Sadohige-Jidori, Siba-Tori, Onaga-Dori, Echigonankin, Hinai, Kinpa, Koeyoshi, and Tomaru) and one imported breed (White Leghorn) were analyzed using eight microsatellite markers that were isolated from a microsatellite DNA-enriched library of chickens (Takahashi et al. 1996). The PCR primers to detect (CA)n repeat length polymorphisms were synthesized based on the sequences of clones, and these markers were typed by PCR amplification and electrophoresis using a DNA sequencer. Since all eight microsatellite markers were polymorphic, genetic distance between the breeds could be calculated based on the frequencies of alleles of the microsatellites and phylogenetic relationships between the breeds could be estimated. Most Japanese native chickens were grouped into three groups that correspond to the origin breeds, Jidori, Shokoku, and Shamo. The results suggest that microsatellite DNA markers are a useful tool for studying the genetic relationships among chicken breeds
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/89.6.543