Quantitative autoradiography using a radioimager based on a multiwire proportional chamber

Determination of the biodistribution of radiopharmaceuticals is an important issue for the evaluation of their performance in diagnosis and therapy. In this study, we evaluated a digital radioimager (RI) based on a multiwire proportional chamber for quantitative autoradiography (AR). The RI allows d...

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Bibliographic Details
Published in:Physics in medicine & biology 1998-12, Vol.43 (12), p.3629-3638
Main Authors: Petegnief, Yolande, Petiet, Anne, Peker, Muammer Can, Bonnin, François, Meulemans, Alain, Guludec, Dominique Le
Format: Article
Language:English
Subjects:
Animals
Applied radiobiology (equipment, dosimetry...)
Autoradiography - instrumentation
Biological and medical sciences
Biological effects of radiation
Data Interpretation, Statistical
Fundamental and applied biological sciences. Psychology
Indium Radioisotopes - analysis
Kidney - chemistry
Male
Myocardium - chemistry
Nuclear Medicine - instrumentation
Radiopharmaceuticals - analysis
Rats
Rats, Wistar
Technetium Tc 99m Dimercaptosuccinic Acid - analysis
Technetium Tc 99m Sestamibi - analysis
Tissues, organs and organisms biophysics
Tomography, Emission-Computed
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Summary:Determination of the biodistribution of radiopharmaceuticals is an important issue for the evaluation of their performance in diagnosis and therapy. In this study, we evaluated a digital radioimager (RI) based on a multiwire proportional chamber for quantitative autoradiography (AR). The RI allows direct detection of electronic emissions of gamma emitters. Its qualitative and quantitative performances were tested on 99mTc and (111)In labelled sections and compared with conventional film AR. Linearity of count rate versus activity was verified over a 104 range of activity. As compared with film AR, a substantial improvement of the detection limit was obtained even for acquisition periods up to 20 times less than film exposure times. We provided the basis for quantitative analysis with tissue equivalent paste standards: the 99mTc and (111)In RI counting efficiencies were respectively 1.19% and 2.35%. We illustrated the respective values of RI and film AR in two rat studies: 99mTc-DMSA in kidney and dual-isotope 99mTc-MIBI and (111)In-antimyosin in heart. Calculated activity concentrations on sections of rat organs confirmed good correlation to gamma counting (deviation less than 12%). We suggest RI as a convenient technique for fast localization of single or dual-isotope tracers and determination of activity distribution.
ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/43/12/018