Peptidyl chemosensors incorporating a fret mechanism for detection of Ni(II)

Hexapeptides incorporating two fluorophores flanking a tripeptide sequence that binds Ni(II) and Cu(II) with high affinity have been synthesized. While Cu(II) quenches the fluorescence of the resulting peptides, coordination of Ni(II) enables enhanced FRET (fluorescent resonance energy transfer) fro...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1998-08, Vol.8 (15), p.1963-1968
Main Authors: Pearce, Dierdre A, Walkup, Grant K, Imperiali, Barbara
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemical and thermal methods
Chemistry
Energy Transfer
Exact sciences and technology
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Nickel - analysis
Oligopeptides - chemistry
Spectrometry, Fluorescence - instrumentation
Spectrophotometry, Ultraviolet
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Summary:Hexapeptides incorporating two fluorophores flanking a tripeptide sequence that binds Ni(II) and Cu(II) with high affinity have been synthesized. While Cu(II) quenches the fluorescence of the resulting peptides, coordination of Ni(II) enables enhanced FRET (fluorescent resonance energy transfer) from one fluorophore to the other. Hexapeptides incorporating two fluorophores flanking a tripeptide sequence that binds Ni(II) and Cu(II) with high affinity have been synthesized. While Cu(II) quenches the fluorescence of the resulting peptides, coordination of Ni(II) enables enhanced FRET (fluorescent resonance energy transfer) from one fluorophore to the other.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(98)00354-0