Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125

• Published in: Applied biochemistry and biotechnology 2008-03, Vol.146 (1-3), p.69-78
• Main Authors: Wagschal, Kurt, Franqui-Espiet, Diana, Lee, Charles C., Robertson, George H., Wong, Dominic W. S.
• Format: Article
• Language: English
• Subjects: Adsorption; Bacillus - enzymology; Bacillus - genetics; Bacillus halodurans; Binding Sites; Biochemistry; Biological and medical sciences; Biotechnology; Chemistry; Chemistry and Materials Science; Cloning; Cloning, Molecular - methods; E coli; Enzyme Activation; Enzyme Stability; Enzymes, Immobilized - chemistry; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Kinetics; Polysaccharides - chemistry; Protein Binding; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Substrate Specificity; Substrates; Triticum aestivum; Xylosidases - chemistry; Xylosidases - genetics; Xylosidases - metabolism
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-007-8055-5

The gene encoding a glycoside hydrolase family 39 xylosidase ( BH1068 ) from the alkaliphile Bacillus halodurans strain C-125 was cloned with a C-terminal His-tag, and the recombinant gene product termed BH1068(His) 6 was expressed in Escherichia coli . Of the artificial substrates tested, BH1068(His) 6 hydrolyzed nitrophenyl derivatives of β- d -xylopyranose, α- l -arabinofuranose, and α- l -arabinopyranose. Deviation from Michaelis−Menten kinetics at higher substrate concentrations indicative of transglycosylation was observed, and k cat and K m values were measured at both low and high substrate concentrations to illuminate the relative propensities to proceed along this alternate reaction pathway. The pH maximum was 6.5, and under the conditions tested, maximal activity was at 47°C, and thermal instability occurred above 45°C. BH1068(His) 6 was inactive on arabinan, hydrolyzed xylooligosaccharides, and released only xylose from oat, wheat, rye, beech, and birch arabinoxylan, and thus, can be classified as a xylosidase with respect to natural substrate specificity. The enzyme was not inhibited by up to 200 mM xylose. The oligomerization state was tetrameric under the size-exclusion chromatography conditions employed.