The laminin α-1 chain derived peptide, AG73, increases fibronectin levels in breast and melanoma cancer cells

Laminin-111 promotes the malignant phenotype, and a 12-mer synthetic peptide (AG73, RKRLQVQLSIRT) from the carboxyl terminus of the α1 chain increases B16F10 melanoma metastasis to the lung and liver. Using an antibody array, fibronectin was identified as an up-regulated protein in B16F10 cells afte...

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Bibliographic Details
Published in:Clinical & experimental metastasis 2008-05, Vol.25 (3), p.241-252
Main Authors: Engbring, Jean A., Hossain, Rydhwana, VanOsdol, Sherilyn J., Kaplan-Singer, Benjamin, Wu, Michael, Hibino, Suguru, Koblinski, Jennifer E.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Breast Neoplasms - drug therapy
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cancer Research
Cell Adhesion
Female
Fibronectins - antagonists & inhibitors
Fibronectins - metabolism
Fluorescent Antibody Technique
Hematology
Immunoblotting
Laminin - pharmacology
Melanoma, Experimental - drug therapy
Melanoma, Experimental - metabolism
Melanoma, Experimental - pathology
Mice
Mice, Inbred C57BL
Mice, Nude
Oncology
Peptide Fragments - pharmacology
Protein Array Analysis
Research Paper
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - pharmacology
Surgical Oncology
Tumor Cells, Cultured
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Summary:Laminin-111 promotes the malignant phenotype, and a 12-mer synthetic peptide (AG73, RKRLQVQLSIRT) from the carboxyl terminus of the α1 chain increases B16F10 melanoma metastasis to the lung and liver. Using an antibody array, fibronectin was identified as an up-regulated protein in B16F10 cells after incubation with this peptide. The increased fibronectin is cell-associated with no increase in soluble fibronectin. The AG73 peptide increased the number and size of bone metastases with both B16F10 melanoma and MDA-231 breast carcinoma cells in an intracardiac injection model. Using siRNA transfection, we found that a reduction in fibronectin expression did not reduce bone metastasis in the presence of the metastasis-promoting peptide AG73. We conclude that the laminin peptide AG73 increases metastasis independently of fibronectin expression.
ISSN:0262-0898
1573-7276
DOI:10.1007/s10585-007-9138-y