LC-MS determination and pharmacokinetic studies of paeonol in rat plasma after administration of different compatibility of Su-Xiao-Xin-Tong prescriptions

• Published in: Biomedical chromatography 2008-05, Vol.22 (5), p.527-534
• Main Authors: Xiao, Yao, Zhang, Ying-hao, Sheng, Yu-xin, Zhang, Jin-lan
• Format: Article
• Language: English
• Subjects: Acetophenones - blood; Acetophenones - chemistry; Acetophenones - pharmacokinetics; Animals; Chromatography, Liquid - methods; Drug Combinations; LC-MS; Medicine, Chinese Traditional; Molecular Structure; paeonol; pharmacokinetic; Rats; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - methods; Su-Xiao-Xin-Tong
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.964

A rapid and specific liquid chromatographic/electrospray ionization mass spectrometric (LC/ESI–MS) method has been developed and validated for the identification and quantification of paeonol in rat plasma. Paeonol and internal standard were isolated from plasma samples by liquid–liquid extraction with chloroform. The chromatographic separation was accomplished on a Zorbax‐SB C18 column (100 × 2.1 mm, 3.5 µm). The mobile phase consisted of acetonitrile and 0.1% aqueous formic acid (64:36) was delivered at a flow rate of 0.2 mL/min. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring mode via electrospray ionization source. Linearity was established for the range of concentrations 0.0525–15.8 µg/mL with a coefficient correlation (r) of 0.9995. The intra‐ and inter‐day precision (RSD%) was lower than 9.34% and accuracy ranged from 93.7 to 102.3%. The lower limit of quantification was 0.0525 µg/mL. The proposed method was used to determine the concentration of paeonol for pharmacokinetic studies. The pharmacokinetics of different compatibility prescriptions of Su‐Xiao‐Xin‐Tong were studied and compared. Copyright © 2008 John Wiley & Sons, Ltd.