Independent Feeding and Metabolic Actions of Orexins in Mice

Orexin-A and orexin-B (OX peptides) are two putative products of a newly discovered secreted protein encoded by a mRNA restricted to neuronal cell bodies of the lateral hypothalamus (LH). Because the activation of the LH can induce changes in energy balance, we wanted to investigate the actions of O...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-12, Vol.253 (2), p.241-245
Main Authors: Lubkin, Margaret, Stricker-Krongrad, Alain
Format: Article
Language:English
Subjects:
Animals
Calorimetry, Indirect
Carrier Proteins - administration & dosage
Carrier Proteins - metabolism
Carrier Proteins - pharmacology
Darkness
Dose-Response Relationship, Drug
Drug Administration Schedule
Eating - drug effects
Energy Metabolism - drug effects
Injections, Intraventricular
Intracellular Signaling Peptides and Proteins
Light
Male
Mice
Mice, Inbred C57BL
Neuropeptides - administration & dosage
Neuropeptides - metabolism
Neuropeptides - pharmacology
Orexins
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Summary:Orexin-A and orexin-B (OX peptides) are two putative products of a newly discovered secreted protein encoded by a mRNA restricted to neuronal cell bodies of the lateral hypothalamus (LH). Because the activation of the LH can induce changes in energy balance, we wanted to investigate the actions of OX peptides on energy metabolism in mice. We injected male C57BL/6J mice with different doses (1, 3, and 10 nmol) of orexin-A and orexin-B into the third ventricle (i3vt). A single i3vt injection of orexin-A 3 h into the light period slightly stimulated feeding at the lowest dose only over the following 4 h (11 ± 09 mg/mouse vs 80 ± 13 mg/mouse,p< 0.05). Orexin-B showed no effects at any dose. We therefore investigated the effects of 3 nmol orexin-A on energy utilization using indirect calorimetry. Single i3vt injection 3 h after light on, or just before dark onset, or in 4-h fasted mice resulted in increases in the metabolic rate. These effects were associated with decreases or increases in the respiratory quotient regarding the time of injection or the underlying metabolic state of the mice. The present findings provide direct evidence that OX peptides are more likely to be involved in the control of energy metabolism than of food intake in mice.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9750