A hydrodynamic approach to the measurement of the permeability of small molecules across artificial membranes

An in situ analytical approach to the measurement of supported liquid membrane permeability is reported. The method consists of a spectrophotometric method to measure transport through a membrane-supported lipid solution, using a rotating-diffusion cell configuration to overcome limits arising from...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2008-05, Vol.133 (5), p.655-659
Main Authors: MOLLOY, Billy-Joe, TAM, Kin Y, WOOD, J. Matthew, DRYFE, Robert A. W
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Diffusion
Electrochemistry - methods
Exact sciences and technology
Hydrophobic and Hydrophilic Interactions
Membranes, Artificial
Permeability
Rotation
Spectrometric and optical methods
Spectrophotometry - instrumentation
Spectrophotometry - methods
Warfarin - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An in situ analytical approach to the measurement of supported liquid membrane permeability is reported. The method consists of a spectrophotometric method to measure transport through a membrane-supported lipid solution, using a rotating-diffusion cell configuration to overcome limits arising from transport through the aqueous solution boundary layer in stationary systems. Rotation frequencies are almost two orders of magnitude higher than those employed previously for rotating-diffusion studies of membrane transport. The method is illustrated with the transport of warfarin [1-(4'-hydroxy-3'-coumarinyl)-1-phenyl-3-butanone]. The use of the rotating-diffusion approach permits accurate calculation of the aqueous phase boundary layer thickness, which has hitherto been treated as an adjustable parameter in studies of membrane permeability. Further, it is shown that the analyte diffusion coefficient can be determined readily using liquid-liquid electrochemistry.
ISSN:0003-2654
1364-5528
DOI:10.1039/b719634b