Development of a multigenic plasmid vector for HCV DNA immunization

HCV viral nucleocapsid protein (C), non-structural protein 3 (NS3) and the envelope glycoproteins E1 and E2 are candidate immune targets for developing anti-HCV DNA vaccine. Nevertheless, the immune response elicited by these antigens often appears weak and/or transient. Different approaches have be...

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Bibliographic Details
Published in:Research in virology (Paris) 1998-09, Vol.149 (5), p.315-319
Main Authors: Papa, S., Rinaldi, M., Mangia, A., Parrella, P., Signori, E., Lombardi, L., Fazio, V.M.
Format: Article
Language:English
Subjects:
Animals
B/T epitopes
Biological and medical sciences
DNA vaccination
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Genetic Vectors
HCV NS3
Hepacivirus - genetics
Hepacivirus - immunology
Hepatitis C - prevention & control
Hepatitis C virus
Interleukin-2 - biosynthesis
Interleukin-2 - genetics
Interleukin-2 - immunology
Mice
Microbiology
Multigenic plasmid vector
murine IL2
Plasmids - genetics
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - immunology
Viral Hepatitis Vaccines - immunology
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - immunology
Virology
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Summary:HCV viral nucleocapsid protein (C), non-structural protein 3 (NS3) and the envelope glycoproteins E1 and E2 are candidate immune targets for developing anti-HCV DNA vaccine. Nevertheless, the immune response elicited by these antigens often appears weak and/or transient. Different approaches have been studied for enhancing and/or modulating the immune response of the DNA vaccine. On the basis of a prototype multigenic plasmid vector constituted of two different transcription cassettes (pRC100), we have developed a plasmid vector that allows the independent and simultaneous expression of murine IL2 and of an antigenic domain of the HCV NS3 C terminus (pRC112-HCV). The highly conserved NS3 region spans from nt 4403 to nt 4829 and contains two putative B and T epitopes. The development of this multigenic plasmid vector may combine the expression and local production of an immunomodulatory molecule (mIL2) together with the possibility of addressing the host immune response to the most immunogenic and conserved epitopes, specifically tailored in the plasmid vector.
ISSN:0923-2516
DOI:10.1016/S0923-2516(99)89012-8