Cholinergic stimulation and nonadrenergic, noncholinergic relaxation of human colonic circular muscle in idiopathic chronic constipation

The aim of our study was to further investigate the pathophysiological mechanism underlying idiopathic chronic constipation (ICC), a disorder of colonic motility. A possible alteration of excitatory and inhibitory neurotransmission and also the role of inhibitory neurotransmitters such as nitric oxi...

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Published in:Digestive diseases and sciences 1998-12, Vol.43 (12), p.2719-2726
Main Authors: MITOLO-CHIEPPA, D, MANSI, G, MEMEO, V, RINALDI, R, MONTAGNANI, M, POTENZA, M. A, GENUALDO, M, SERIO, M, MITOLO, C. I, RINALDI, M, ALTOMARE, D. F
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Adenosine Triphosphate - physiology
Adult
Atropine - pharmacology
Biological and medical sciences
Cholinergic Fibers - physiology
Chronic Disease
Colon - innervation
Colon - physiology
Constipation - physiopathology
Electric Stimulation
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal Motility - physiology
Guanethidine - pharmacology
Humans
In Vitro Techniques
Male
Medical sciences
Muscle Contraction - physiology
Muscle Relaxation - physiology
Muscle, Smooth - physiology
Nitric Oxide - physiology
Other diseases. Semiology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Substance P - pharmacology
Vasoactive Intestinal Peptide - physiology
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Summary:The aim of our study was to further investigate the pathophysiological mechanism underlying idiopathic chronic constipation (ICC), a disorder of colonic motility. A possible alteration of excitatory and inhibitory neurotransmission and also the role of inhibitory neurotransmitters such as nitric oxide (NO), 5'-adenosine triphosphate (ATP), and vasoactive intestinal peptide (VIP) has been evaluated on preparations of distal colon from patients with or without ICC. The isometric tension was recorded from isolated circular muscle strips of both experimental groups during pharmacological and electrical field stimulation (EFS). The contractile response obtained by acetylcholine (ACh 20 microM), EFS (20 Hz, 20 V, 1 msec, pulse trains lasting 1 min) and substance P (SP 1 microM) was significantly lower in ICC than in control preparations. The effect of inhibitory nonadrenergic, noncholinergic innervation was evaluated using EFS at low frequencies (0.5-8 Hz), after cholinergic and sympathetic blockade with atropine (3 microM) and guanethidine (3 microM). The maximum relaxation value expressed as percentage of inhibition of SP-induced contraction was significantly higher in ICC than in control preparations (87+/-2.4 and 67+/-6.3, respectively; P
ISSN:0163-2116
1573-2568
DOI:10.1023/a:1026615730533