Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy

The ideal in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these cr...

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Bibliographic Details
Published in:Matrix biology 1998-11, Vol.17 (7), p.513-523
Main Authors: Potter, K., Butler, J.J., Adams, C., Fishbein, K.W., McFarland, E.W., Horton, W.E., Spencer, R.G.S.
Format: Article
Language:English
Subjects:
Animals
bioreactor
Cartilage - chemistry
Cartilage - cytology
Cartilage - growth & development
cartilage formation
Chick Embryo
chondrocytes
Collagen - genetics
Culture Techniques - instrumentation
Culture Techniques - methods
Extracellular Matrix
Magnetic Resonance Spectroscopy
nuclear magnetic resonance
RNA, Messenger - analysis
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Summary:The ideal in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these criteria. After injection with embryonic chick sternal chondrocytes, neocartilage is elaborated around the hollow fibers, reaching a thickness of up to a millimeter after four weeks of growth. This process was monitored over time with nuclear magnetic resonance (NMR) microimaging and correlative biochemical and histologic analyses. Tissue volume and cellularity increased greatly during development. This was accompanied by changes in magnetic resonance properties consistent with increased macromolecular content. Further, tissue heterogeneity, observed as regional variations in cell size in histologic sections, was also observed in quantitative NMR images.
ISSN:0945-053X
1569-1802
DOI:10.1016/S0945-053X(98)90099-3