Loading…

Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa

BACKGROUND Oxidative stress induced by reactive oxygen species (ROS) is associated with an impaired fertilization ability of spermatozoa. We investigated the effects of adding antioxidants to a sperm preparation medium on the functional parameters of the spermatozoa. METHODS Spermatozoa were washed...

Full description

Saved in:
Bibliographic Details
Published in:Human reproduction (Oxford) 2008-05, Vol.23 (5), p.1023-1028
Main Authors: Chi, H.J., Kim, J.H., Ryu, C.S., Lee, J.Y., Park, J.S., Chung, D.Y., Choi, S.Y., Kim, M.H., Chun, E.K., Roh, S.I.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3
cites cdi_FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3
container_end_page 1028
container_issue 5
container_start_page 1023
container_title Human reproduction (Oxford)
container_volume 23
creator Chi, H.J.
Kim, J.H.
Ryu, C.S.
Lee, J.Y.
Park, J.S.
Chung, D.Y.
Choi, S.Y.
Kim, M.H.
Chun, E.K.
Roh, S.I.
description BACKGROUND Oxidative stress induced by reactive oxygen species (ROS) is associated with an impaired fertilization ability of spermatozoa. We investigated the effects of adding antioxidants to a sperm preparation medium on the functional parameters of the spermatozoa. METHODS Spermatozoa were washed with Ham's F-10 media containing the antioxidants, ethylenediaminetetraacetic acid (EDTA) and catalase, at various concentrations, and then the ROS levels in sperm suspensions, and the forward motility, acrosome reaction, DNA integrity and lipid peroxidation of the spermatozoa were assessed. RESULTS The ROS levels were significantly lower in sperm suspensions washed with the antioxidants (196∼312 rlu; relative light units) than in control sperm (604 rlu, P < 0.05). The addition of 10 µM EDTA to the sperm preparation medium significantly improved the motility of the spermatozoa compared with the control group, the groups containing EDTA at other concentrations and the groups containing catalase. Catalase significantly increased the acrosome reaction rate of the spermatozoa. Both EDTA and catalase significantly decreased the DNA fragmentation rate of the spermatozoa. However, the antioxidants did not reduce lipid peroxidation. CONCLUSIONS Supplementing sperm preparation medium with EDTA or catalase significantly improved the overall functional parameters of the spermatozoa by reducing the ROS levels.
doi_str_mv 10.1093/humrep/den060
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69129322</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/humrep/den060</oup_id><sourcerecordid>1468019121</sourcerecordid><originalsourceid>FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3</originalsourceid><addsrcrecordid>eNqFkc1rFTEUxYMo9lldupVBqLgZm--ZLKVVX6FUCxXETcjL3NHUmWRMMlL715vXGSq4cXUvl9899yQHoecEvyFYsePv8xhhOu7AY4kfoA3hEteUCfwQbTCVbU2IJAfoSUrXGJe2lY_RAWkZFW3LN-j2UwwZbHa_oIK-L10V-sr47MKN60qt0jxNA4zgsylDXzlfpQniWE_lronLcITOzWNlvhnnU5HY795pphwhpf1SMWrWVZPDbTBP0aPeDAmerfUQfX7_7upkW59__HB28va8toLyXHOFG6u6VvQKE95RTgU2gjamAwZS8rYvr-kENxZbSRXZEWFZ23DR0HbHBbBD9GrRnWL4OUPKenTJwjAYD2FOWipCFaO0gC__Aa_DHH3xpikhimNGZIHqBbIxpBSh11N0o4m_NcF6n4heEtFLIoV_sYrOu_JLf-k1ggIcrYBJ1gx9NN66dM9RzGjTMFW41wsX5um_N1ePLmW4uYdN_KFlwxqht1--6itxcXpxKbb6kv0BXq604w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>211940316</pqid></control><display><type>article</type><title>Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa</title><source>Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)</source><creator>Chi, H.J. ; Kim, J.H. ; Ryu, C.S. ; Lee, J.Y. ; Park, J.S. ; Chung, D.Y. ; Choi, S.Y. ; Kim, M.H. ; Chun, E.K. ; Roh, S.I.</creator><creatorcontrib>Chi, H.J. ; Kim, J.H. ; Ryu, C.S. ; Lee, J.Y. ; Park, J.S. ; Chung, D.Y. ; Choi, S.Y. ; Kim, M.H. ; Chun, E.K. ; Roh, S.I.</creatorcontrib><description>BACKGROUND Oxidative stress induced by reactive oxygen species (ROS) is associated with an impaired fertilization ability of spermatozoa. We investigated the effects of adding antioxidants to a sperm preparation medium on the functional parameters of the spermatozoa. METHODS Spermatozoa were washed with Ham's F-10 media containing the antioxidants, ethylenediaminetetraacetic acid (EDTA) and catalase, at various concentrations, and then the ROS levels in sperm suspensions, and the forward motility, acrosome reaction, DNA integrity and lipid peroxidation of the spermatozoa were assessed. RESULTS The ROS levels were significantly lower in sperm suspensions washed with the antioxidants (196∼312 rlu; relative light units) than in control sperm (604 rlu, P &lt; 0.05). The addition of 10 µM EDTA to the sperm preparation medium significantly improved the motility of the spermatozoa compared with the control group, the groups containing EDTA at other concentrations and the groups containing catalase. Catalase significantly increased the acrosome reaction rate of the spermatozoa. Both EDTA and catalase significantly decreased the DNA fragmentation rate of the spermatozoa. However, the antioxidants did not reduce lipid peroxidation. CONCLUSIONS Supplementing sperm preparation medium with EDTA or catalase significantly improved the overall functional parameters of the spermatozoa by reducing the ROS levels.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/den060</identifier><identifier>PMID: 18325884</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Acrosome Reaction - drug effects ; antioxidant ; Antioxidants - pharmacology ; Biological and medical sciences ; Catalase - pharmacology ; DNA fragmentation ; DNA Fragmentation - drug effects ; Edetic Acid - pharmacology ; Gynecology. Andrology. Obstetrics ; Humans ; Lipid Peroxidation - drug effects ; Male ; Medical sciences ; motility ; oxidative stress ; Oxidative Stress - drug effects ; Reactive Oxygen Species - metabolism ; Sperm Motility - drug effects ; spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology</subject><ispartof>Human reproduction (Oxford), 2008-05, Vol.23 (5), p.1023-1028</ispartof><rights>The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org 2008</rights><rights>2008 INIST-CNRS</rights><rights>The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3</citedby><cites>FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=20327739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18325884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chi, H.J.</creatorcontrib><creatorcontrib>Kim, J.H.</creatorcontrib><creatorcontrib>Ryu, C.S.</creatorcontrib><creatorcontrib>Lee, J.Y.</creatorcontrib><creatorcontrib>Park, J.S.</creatorcontrib><creatorcontrib>Chung, D.Y.</creatorcontrib><creatorcontrib>Choi, S.Y.</creatorcontrib><creatorcontrib>Kim, M.H.</creatorcontrib><creatorcontrib>Chun, E.K.</creatorcontrib><creatorcontrib>Roh, S.I.</creatorcontrib><title>Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>BACKGROUND Oxidative stress induced by reactive oxygen species (ROS) is associated with an impaired fertilization ability of spermatozoa. We investigated the effects of adding antioxidants to a sperm preparation medium on the functional parameters of the spermatozoa. METHODS Spermatozoa were washed with Ham's F-10 media containing the antioxidants, ethylenediaminetetraacetic acid (EDTA) and catalase, at various concentrations, and then the ROS levels in sperm suspensions, and the forward motility, acrosome reaction, DNA integrity and lipid peroxidation of the spermatozoa were assessed. RESULTS The ROS levels were significantly lower in sperm suspensions washed with the antioxidants (196∼312 rlu; relative light units) than in control sperm (604 rlu, P &lt; 0.05). The addition of 10 µM EDTA to the sperm preparation medium significantly improved the motility of the spermatozoa compared with the control group, the groups containing EDTA at other concentrations and the groups containing catalase. Catalase significantly increased the acrosome reaction rate of the spermatozoa. Both EDTA and catalase significantly decreased the DNA fragmentation rate of the spermatozoa. However, the antioxidants did not reduce lipid peroxidation. CONCLUSIONS Supplementing sperm preparation medium with EDTA or catalase significantly improved the overall functional parameters of the spermatozoa by reducing the ROS levels.</description><subject>Acrosome Reaction - drug effects</subject><subject>antioxidant</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Catalase - pharmacology</subject><subject>DNA fragmentation</subject><subject>DNA Fragmentation - drug effects</subject><subject>Edetic Acid - pharmacology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>motility</subject><subject>oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Sperm Motility - drug effects</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkc1rFTEUxYMo9lldupVBqLgZm--ZLKVVX6FUCxXETcjL3NHUmWRMMlL715vXGSq4cXUvl9899yQHoecEvyFYsePv8xhhOu7AY4kfoA3hEteUCfwQbTCVbU2IJAfoSUrXGJe2lY_RAWkZFW3LN-j2UwwZbHa_oIK-L10V-sr47MKN60qt0jxNA4zgsylDXzlfpQniWE_lronLcITOzWNlvhnnU5HY795pphwhpf1SMWrWVZPDbTBP0aPeDAmerfUQfX7_7upkW59__HB28va8toLyXHOFG6u6VvQKE95RTgU2gjamAwZS8rYvr-kENxZbSRXZEWFZ23DR0HbHBbBD9GrRnWL4OUPKenTJwjAYD2FOWipCFaO0gC__Aa_DHH3xpikhimNGZIHqBbIxpBSh11N0o4m_NcF6n4heEtFLIoV_sYrOu_JLf-k1ggIcrYBJ1gx9NN66dM9RzGjTMFW41wsX5um_N1ePLmW4uYdN_KFlwxqht1--6itxcXpxKbb6kv0BXq604w</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Chi, H.J.</creator><creator>Kim, J.H.</creator><creator>Ryu, C.S.</creator><creator>Lee, J.Y.</creator><creator>Park, J.S.</creator><creator>Chung, D.Y.</creator><creator>Choi, S.Y.</creator><creator>Kim, M.H.</creator><creator>Chun, E.K.</creator><creator>Roh, S.I.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa</title><author>Chi, H.J. ; Kim, J.H. ; Ryu, C.S. ; Lee, J.Y. ; Park, J.S. ; Chung, D.Y. ; Choi, S.Y. ; Kim, M.H. ; Chun, E.K. ; Roh, S.I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acrosome Reaction - drug effects</topic><topic>antioxidant</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Catalase - pharmacology</topic><topic>DNA fragmentation</topic><topic>DNA Fragmentation - drug effects</topic><topic>Edetic Acid - pharmacology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>motility</topic><topic>oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Sperm Motility - drug effects</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chi, H.J.</creatorcontrib><creatorcontrib>Kim, J.H.</creatorcontrib><creatorcontrib>Ryu, C.S.</creatorcontrib><creatorcontrib>Lee, J.Y.</creatorcontrib><creatorcontrib>Park, J.S.</creatorcontrib><creatorcontrib>Chung, D.Y.</creatorcontrib><creatorcontrib>Choi, S.Y.</creatorcontrib><creatorcontrib>Kim, M.H.</creatorcontrib><creatorcontrib>Chun, E.K.</creatorcontrib><creatorcontrib>Roh, S.I.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chi, H.J.</au><au>Kim, J.H.</au><au>Ryu, C.S.</au><au>Lee, J.Y.</au><au>Park, J.S.</au><au>Chung, D.Y.</au><au>Choi, S.Y.</au><au>Kim, M.H.</au><au>Chun, E.K.</au><au>Roh, S.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>23</volume><issue>5</issue><spage>1023</spage><epage>1028</epage><pages>1023-1028</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND Oxidative stress induced by reactive oxygen species (ROS) is associated with an impaired fertilization ability of spermatozoa. We investigated the effects of adding antioxidants to a sperm preparation medium on the functional parameters of the spermatozoa. METHODS Spermatozoa were washed with Ham's F-10 media containing the antioxidants, ethylenediaminetetraacetic acid (EDTA) and catalase, at various concentrations, and then the ROS levels in sperm suspensions, and the forward motility, acrosome reaction, DNA integrity and lipid peroxidation of the spermatozoa were assessed. RESULTS The ROS levels were significantly lower in sperm suspensions washed with the antioxidants (196∼312 rlu; relative light units) than in control sperm (604 rlu, P &lt; 0.05). The addition of 10 µM EDTA to the sperm preparation medium significantly improved the motility of the spermatozoa compared with the control group, the groups containing EDTA at other concentrations and the groups containing catalase. Catalase significantly increased the acrosome reaction rate of the spermatozoa. Both EDTA and catalase significantly decreased the DNA fragmentation rate of the spermatozoa. However, the antioxidants did not reduce lipid peroxidation. CONCLUSIONS Supplementing sperm preparation medium with EDTA or catalase significantly improved the overall functional parameters of the spermatozoa by reducing the ROS levels.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>18325884</pmid><doi>10.1093/humrep/den060</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0268-1161
ispartof Human reproduction (Oxford), 2008-05, Vol.23 (5), p.1023-1028
issn 0268-1161
1460-2350
language eng
recordid cdi_proquest_miscellaneous_69129322
source Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)
subjects Acrosome Reaction - drug effects
antioxidant
Antioxidants - pharmacology
Biological and medical sciences
Catalase - pharmacology
DNA fragmentation
DNA Fragmentation - drug effects
Edetic Acid - pharmacology
Gynecology. Andrology. Obstetrics
Humans
Lipid Peroxidation - drug effects
Male
Medical sciences
motility
oxidative stress
Oxidative Stress - drug effects
Reactive Oxygen Species - metabolism
Sperm Motility - drug effects
spermatozoa
Spermatozoa - drug effects
Spermatozoa - physiology
title Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A55%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20effect%20of%20antioxidant%20supplementation%20in%20sperm-preparation%20medium%20against%20oxidative%20stress%20in%20human%20spermatozoa&rft.jtitle=Human%20reproduction%20(Oxford)&rft.au=Chi,%20H.J.&rft.date=2008-05-01&rft.volume=23&rft.issue=5&rft.spage=1023&rft.epage=1028&rft.pages=1023-1028&rft.issn=0268-1161&rft.eissn=1460-2350&rft.coden=HUREEE&rft_id=info:doi/10.1093/humrep/den060&rft_dat=%3Cproquest_cross%3E1468019121%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c524t-4907c9d85f9014d24250a527ade3e6648f183d54ac0c6291b15c38745728b45e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=211940316&rft_id=info:pmid/18325884&rft_oup_id=10.1093/humrep/den060&rfr_iscdi=true