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Analysis of the exponential character of single molecule rotational correlation functions for large and small fluorescence collection angles
Coarse-grained molecular dynamics simulations and single molecule fluorescence microscopy experiments have been performed in order to investigate the influence of the numerical aperture (NA) of the microscope objective on the exponential character of the rotational correlation functions of probes em...
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Published in: | The Journal of chemical physics 2008-04, Vol.128 (15), p.154515-154515-8 |
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container_end_page | 154515-8 |
container_issue | 15 |
container_start_page | 154515 |
container_title | The Journal of chemical physics |
container_volume | 128 |
creator | Vallée, R. A. L. Rohand, T. Boens, N. Dehaen, W. Hinze, G. Basché, T. |
description | Coarse-grained molecular dynamics simulations and single molecule fluorescence microscopy experiments have been performed in order to investigate the influence of the numerical aperture (NA) of the microscope objective on the exponential character of the rotational correlation functions of probes embedded in complex matrices. The results obtained by using either a dry lens
(
NA
=
0.95
)
or an oil objective
(
NA
=
1.4
)
show that, in the moderately (simulations) and deeply (experiment) supercooled melts, the rotational (linear dichroism) correlation functions of the single molecules (SMs) exhibit a nonexponential character. Furthermore, by fitting Kohlrausch-Williams-Watt functions to the correlation curves, the stretching parameters turn out to be very similar for both types of objectives. Our results demonstrate that the nonexponentiality is intrinsic to the complex rotational dynamics of the SM in the supercooled solid and point to the validity of the use of a high NA dry lens to perform such experiments. |
doi_str_mv | 10.1063/1.2904557 |
format | article |
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(
NA
=
0.95
)
or an oil objective
(
NA
=
1.4
)
show that, in the moderately (simulations) and deeply (experiment) supercooled melts, the rotational (linear dichroism) correlation functions of the single molecules (SMs) exhibit a nonexponential character. Furthermore, by fitting Kohlrausch-Williams-Watt functions to the correlation curves, the stretching parameters turn out to be very similar for both types of objectives. Our results demonstrate that the nonexponentiality is intrinsic to the complex rotational dynamics of the SM in the supercooled solid and point to the validity of the use of a high NA dry lens to perform such experiments.</description><identifier>ISSN: 0021-9606</identifier><identifier>EISSN: 1089-7690</identifier><identifier>DOI: 10.1063/1.2904557</identifier><identifier>PMID: 18433243</identifier><identifier>CODEN: JCPSA6</identifier><language>eng</language><publisher>United States: American Institute of Physics</publisher><ispartof>The Journal of chemical physics, 2008-04, Vol.128 (15), p.154515-154515-8</ispartof><rights>2008 American Institute of Physics</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-87faeed95c5d3b17ed34c76e3cba44bd3703f8792cb118f1eef89c1433a2a2e43</citedby><cites>FETCH-LOGICAL-c338t-87faeed95c5d3b17ed34c76e3cba44bd3703f8792cb118f1eef89c1433a2a2e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,779,781,792,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18433243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vallée, R. A. L.</creatorcontrib><creatorcontrib>Rohand, T.</creatorcontrib><creatorcontrib>Boens, N.</creatorcontrib><creatorcontrib>Dehaen, W.</creatorcontrib><creatorcontrib>Hinze, G.</creatorcontrib><creatorcontrib>Basché, T.</creatorcontrib><title>Analysis of the exponential character of single molecule rotational correlation functions for large and small fluorescence collection angles</title><title>The Journal of chemical physics</title><addtitle>J Chem Phys</addtitle><description>Coarse-grained molecular dynamics simulations and single molecule fluorescence microscopy experiments have been performed in order to investigate the influence of the numerical aperture (NA) of the microscope objective on the exponential character of the rotational correlation functions of probes embedded in complex matrices. The results obtained by using either a dry lens
(
NA
=
0.95
)
or an oil objective
(
NA
=
1.4
)
show that, in the moderately (simulations) and deeply (experiment) supercooled melts, the rotational (linear dichroism) correlation functions of the single molecules (SMs) exhibit a nonexponential character. Furthermore, by fitting Kohlrausch-Williams-Watt functions to the correlation curves, the stretching parameters turn out to be very similar for both types of objectives. Our results demonstrate that the nonexponentiality is intrinsic to the complex rotational dynamics of the SM in the supercooled solid and point to the validity of the use of a high NA dry lens to perform such experiments.</description><issn>0021-9606</issn><issn>1089-7690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp1kc1KxDAUhYMozji68AUkK8FFNWn6uxGGwT8YcKPrkKY3M5U0GZMWnHfwoU2nFVeu7r3ku4ebcxC6pOSWkozd0du4JEma5kdoTklRRnlWkmM0JySmUZmRbIbOvP8ghNA8Tk7RjBYJY3HC5uh7aYTe-8Zjq3C3BQxfO2vAdI3QWG6FE7IDNzz6xmw04NZqkH1onO1E11gzcNY50IcJq97IofFYWYe1cBvAwtTYt0JrrHRvHXgJRkJY00HrsCUGbX-OTpTQHi6mukDvjw9vq-do_fr0slquI8lY0UVFrgRAXaYyrVlFc6hZIvMMmKxEklQ1ywlTRV7GsqK0UBRAFaWk4csiFjEkbIGuR92ds589-I63TbhJa2HA9p5nJWWkCM4u0M0ISme9d6D4zjWtcHtOCR-s55RP1gf2ahLtqxbqP3LyOgD3I-BlM1r3v9pvKtwqHlLhQyrsB_pbl_M</recordid><startdate>20080421</startdate><enddate>20080421</enddate><creator>Vallée, R. A. L.</creator><creator>Rohand, T.</creator><creator>Boens, N.</creator><creator>Dehaen, W.</creator><creator>Hinze, G.</creator><creator>Basché, T.</creator><general>American Institute of Physics</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080421</creationdate><title>Analysis of the exponential character of single molecule rotational correlation functions for large and small fluorescence collection angles</title><author>Vallée, R. A. L. ; Rohand, T. ; Boens, N. ; Dehaen, W. ; Hinze, G. ; Basché, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-87faeed95c5d3b17ed34c76e3cba44bd3703f8792cb118f1eef89c1433a2a2e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vallée, R. A. L.</creatorcontrib><creatorcontrib>Rohand, T.</creatorcontrib><creatorcontrib>Boens, N.</creatorcontrib><creatorcontrib>Dehaen, W.</creatorcontrib><creatorcontrib>Hinze, G.</creatorcontrib><creatorcontrib>Basché, T.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of chemical physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vallée, R. A. L.</au><au>Rohand, T.</au><au>Boens, N.</au><au>Dehaen, W.</au><au>Hinze, G.</au><au>Basché, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the exponential character of single molecule rotational correlation functions for large and small fluorescence collection angles</atitle><jtitle>The Journal of chemical physics</jtitle><addtitle>J Chem Phys</addtitle><date>2008-04-21</date><risdate>2008</risdate><volume>128</volume><issue>15</issue><spage>154515</spage><epage>154515-8</epage><pages>154515-154515-8</pages><issn>0021-9606</issn><eissn>1089-7690</eissn><coden>JCPSA6</coden><abstract>Coarse-grained molecular dynamics simulations and single molecule fluorescence microscopy experiments have been performed in order to investigate the influence of the numerical aperture (NA) of the microscope objective on the exponential character of the rotational correlation functions of probes embedded in complex matrices. The results obtained by using either a dry lens
(
NA
=
0.95
)
or an oil objective
(
NA
=
1.4
)
show that, in the moderately (simulations) and deeply (experiment) supercooled melts, the rotational (linear dichroism) correlation functions of the single molecules (SMs) exhibit a nonexponential character. Furthermore, by fitting Kohlrausch-Williams-Watt functions to the correlation curves, the stretching parameters turn out to be very similar for both types of objectives. Our results demonstrate that the nonexponentiality is intrinsic to the complex rotational dynamics of the SM in the supercooled solid and point to the validity of the use of a high NA dry lens to perform such experiments.</abstract><cop>United States</cop><pub>American Institute of Physics</pub><pmid>18433243</pmid><doi>10.1063/1.2904557</doi><tpages>1</tpages></addata></record> |
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source | American Institute of Physics:Jisc Collections:Transitional Journals Agreement 2021-23 (Reading list); American Institute of Physics(アメリカ物理学協会) |
title | Analysis of the exponential character of single molecule rotational correlation functions for large and small fluorescence collection angles |
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