Determination of the enantiomeric purity of (−) terbutaline by capillary electrophoresis using cyclodextrins as chiral selectors in a polyethylene glycol gel

A method was developed for determination of the enantiomeric purity of the therapeutic-pharmacological active (−)-enantiomer of terbutaline using cyclodextrins as a chiral selector dissolved in a removable liquid polyethylene glycol gel by use of capillary electrophoresis. The effect of temperature,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1998-09, Vol.17 (6), p.1047-1056
Main Authors: de Boer, Theo, Ensing, Kees
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Chemistry Techniques, Analytical - methods
Cyclodextrins
Cyclodextrins - chemistry
Drug Contamination
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Enantiomeric purity
Polyethylene glycol
Polyethylene Glycols - chemistry
Stereoisomerism
Temperature
Terbutaline - chemistry
Terbutaline hemisulphate
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method was developed for determination of the enantiomeric purity of the therapeutic-pharmacological active (−)-enantiomer of terbutaline using cyclodextrins as a chiral selector dissolved in a removable liquid polyethylene glycol gel by use of capillary electrophoresis. The effect of temperature, type and concentration of polyethylene glycol and cyclodextrins was studied on the resolution between the two enantiomers. Best results were obtained with 10 mM hydroxyethyl- β-cyclodextrin dissolved in a 10% polyethylene glycol-2000 solution at 15°C. Under these conditions, an impurity of 0.1% (distomer/eutomer) can be readily detected.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(98)00070-3