Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo

We established a xenograft line of human adrenocortical carcinoma (ADR‐1), and analyzed the hyper‐aldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25‐year‐old woman were subcutaneously inoculated into nude mice (BALB/c‐nu/nu) followed by serial passages in vi...

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Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1998-11, Vol.106 (7-12), p.1056-1060
Main Authors: YAMAZAKI, HITOSHI, ABE, YOSHIYUKI, KATOH, YUKO, SAWA, NOBUKO, OHNISHI, YASUYUKI, TANAKA, YUJI, SASANO, HIRONOBU, OSHIKA, YOSHIRO, TOKUNAGA, TESTUJI, KIJIMA, HIROSHI, TAMAOKI, NORIKAZU, NAKAMURA, MASATO, UEYAMA, YOSHITO
Format: Article
Language:English
Subjects:
Adrenal Gland Neoplasms - pathology
Adrenal Gland Neoplasms - physiopathology
Adrenals. Adrenal axis. Renin-angiotensin system (diseases)
adrenocortical carcinoma
Adult
Aldosterone
Animals
Biological and medical sciences
Blood Pressure
Carcinoma - pathology
Carcinoma - physiopathology
Endocrinopathies
Female
Humans
Hyperaldosteronism - physiopathology
Medical sciences
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Neoplasms, Experimental - pathology
Neoplasms, Experimental - physiopathology
Non tumoral diseases. Target tissue resistance. Benign neoplasms
Rats
Transplantation, Heterologous
xenograft
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Summary:We established a xenograft line of human adrenocortical carcinoma (ADR‐1), and analyzed the hyper‐aldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25‐year‐old woman were subcutaneously inoculated into nude mice (BALB/c‐nu/nu) followed by serial passages in vivo. ADR‐1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/1), but did not show hypertension. The nude rat (F344‐rnu/rnu) bearing ADR‐1 showed hyperaldosteronism (serum aldosterone 3320 ± 1420 pg/ml; control 191 ± 130 pg/ml) and hypokalemia (serum K 3.4 ± 0.4 mEq/1; control 5.2 ± 1.0 mEq/1) in vivo, and hypertension was not obvious. ADR‐1 was shown immunohistochemically to retain production of human‐specific corticosteroid synthetase. The xenograft ADR‐1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.
ISSN:0903-4641
1600-0463
DOI:10.1111/j.1699-0463.1998.tb00258.x