Comparison of various diagnostic methods in characterizing Newcastle disease virus isolates from Desi chickens

Eleven Newcastle disease viruses (NDV), isolated from apparently healthy and ailing Desi chickens were subjected to both conventional and modern characterization techniques. The virulence and strain differentiating experiments placed 10 isolates in the velogenic group and one in the mesogenic group....

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Bibliographic Details
Published in:Tropical animal health and production 1998-10, Vol.30 (5), p.287-293
Main Authors: Raghavan, V.S, Kumanan, K, Thirumurugan, G, Nachimuthu, K
Format: Article
Language:English
Subjects:
Animals
Cattle
Cell Line
Chickens
Cloaca - virology
Cytopathogenic Effect, Viral
cytopathogenicity
DNA probes
DNA Probes - chemistry
Eggs
hemagglutination
Hemagglutination Tests - veterinary
India
laboratory techniques
native livestock
Neutralization Tests - veterinary
Newcastle Disease - diagnosis
Newcastle Disease - parasitology
Newcastle disease virus
Newcastle disease virus - classification
Newcastle disease virus - pathogenicity
Nucleic Acid Hybridization
rapid methods
RNA, Viral - chemistry
strain differences
Virulence
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Summary:Eleven Newcastle disease viruses (NDV), isolated from apparently healthy and ailing Desi chickens were subjected to both conventional and modern characterization techniques. The virulence and strain differentiating experiments placed 10 isolates in the velogenic group and one in the mesogenic group. In MDBK cells, 9 isolates produced characteristic cytopathogenic effects up to 5 and 2 up to 3 passages. Molecular characterization with a 21-mer oligonucleotide probe placed all the isolates in the velogenic/mesogenic group. The results of this study clearly indicated that the isolates obtained are either velogenic or mesogenic but not lentogenic.
ISSN:0049-4747
1573-7438
DOI:10.1023/A:1005034719475