Effect of estrogen receptor beta on the osteoblastic differentiation function of human periodontal ligament cells

Abstract Objective To investigate the effect of estrogen receptor beta (ERβ) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro. Design We employed a short interferi...

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Bibliographic Details
Published in:Archives of oral biology 2008-06, Vol.53 (6), p.553-557
Main Authors: Liang, Li, Yu, Ji-feng, Wang, Yan, Wang, Guang, Ding, Yin
Format: Article
Language:English
Subjects:
Advanced Basic Science
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Cell Differentiation - drug effects
Child
Dentistry
Estradiol - pharmacology
Estrogen
Estrogen receptor beta
Estrogen Receptor beta - physiology
Estrogens - pharmacology
Humans
Osteoblasts - metabolism
Osteocalcin
Osteocalcin - metabolism
Periodontal ligament
Periodontal Ligament - metabolism
siRNA
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Summary:Abstract Objective To investigate the effect of estrogen receptor beta (ERβ) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro. Design We employed a short interfering RNA (siRNA) technique to inhibit ERβ expression in hPDL cells; the cells were cultured with a saturating concentration of 17β-estradiol (10−7 M). ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit. Results It was shown that estradiol significantly enhanced the ALP activity and the production of OCN in hPDL cells. However, the ALP activity and the production of OCN in hPDL-siERβ cells were not significantly changed after estradiol treatment. Conclusions These results indicate that ERβ may play important roles in estrogen-induced effects on osteoblastic differentiation function of PDL cells and estrogen influences the bone formation capacity of PDL cells mainly via ERβ.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2007.12.011