Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity

Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.

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Bibliographic Details
Published in:Molecular bioSystems 2008-01, Vol.4 (3), p.246-248
Main Authors: Zha, Wenjuan, Rubin-Pitel, Sheryl B, Zhao, Huimin
Format: Article
Language:English
Subjects:
Acyltransferases - chemistry
Acyltransferases - genetics
Acyltransferases - metabolism
Amino Acid Sequence
Catalysis
Databases, Protein
Directed Molecular Evolution
Genetic Variation - genetics
Kinetics
Molecular Sequence Data
Mutation - genetics
Protein Engineering
Pseudomonas fluorescens - enzymology
Pseudomonas fluorescens - genetics
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Summary:Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
ISSN:1742-206X
1742-2051
DOI:10.1039/b717705d