Screening Autoantibody Profiles in Systemic Rheumatic Disease with a Diagnostic Protein Microarray That Uses a Filtration-Assisted Nanodot Array Luminometric Immunoassay (NALIA)

We developed a cost-efficient modular system for multiplex analysis of the multiple autoantibodies that characterize systemic rheumatoid diseases. The nanodot array luminometric immunoassay (NALIA) system consists of conventional 96-well membrane-bottomed plates in which antigens or antibodies are a...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2008-05, Vol.54 (5), p.883-890
Main Authors: McBride, Jeffrey D, Gabriel, Francis Guy, Fordham, John, Kolind, Torsten, Barcenas-Morales, Gabriela, Isenberg, David A, Swana, Marlene, Delves, Peter J, Lund, Torben, Cree, Ian A, Roitt, Ivan M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Antigens
Autoantibodies - blood
Biological and medical sciences
Cellulose acetate
Chemiluminescence
Disease
Fundamental and applied biological sciences. Psychology
Humans
Immunoassay
Immunology
Investigative techniques, diagnostic techniques (general aspects)
Life sciences
Luminescent Measurements
Lupus
Medical sciences
Medical screening
Nanostructures
Patients
Protein Array Analysis
Proteins
Reagents
Rheumatic diseases
Rheumatic Diseases - immunology
Rheumatology
Sensitivity and Specificity
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Summary:We developed a cost-efficient modular system for multiplex analysis of the multiple autoantibodies that characterize systemic rheumatoid diseases. The nanodot array luminometric immunoassay (NALIA) system consists of conventional 96-well membrane-bottomed plates in which antigens or antibodies are adsorbed onto the underside of the membrane. Current arrays use a 5 x 5 format (25 dots/well), which allows 10 analytes to be measured in duplicate: double-stranded DNA (dsDNA), centromere protein B (CENP-B), PCNA, Sm, Sm ribonucleoprotein (Sm-RNP), U1-snRNP, Scl70, SSA/Ro, SSB/La, Jo-1, and controls. The test fluid, control sera, and subsequent reagents are drawn through the membrane. The captured analytes are quantified by monitoring chemiluminescence with a charge-coupled device (CCD) and analyzed with commercial array software. The assay can detect
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2007.098418