A Quantitative Assay for Angiogenesis of Cultured Choroidal Tissues in Streptozotocin-Diabetic Wistar and Spontaneously Diabetic GK Rats

Angiogenesis of cultured choroids was quantitatively assayed in spontaneously diabetic GK and a bolus-treated streptozotocin (STZ)-diabetic Wistar rats. The number and total length of microvessels budded from cultured choroidal explants were measured to use as angiogenic indices. Both indices in 10-...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1998, Vol.78(4), pp.471-478
Main Authors: Shinjiro, Kobayashi, Mizuki, Fukuta, Hitoshi, Kontani, Sumino, Yanagita, Ikuko, Kimura
Format: Article
Language:English
Subjects:
Aging - physiology
Animals
Capillaries - drug effects
Capillaries - physiopathology
Cattle
Choroid - drug effects
Choroid - physiopathology
Choroidal angiogenesis
Culture Media - chemistry
Culture Media - pharmacology
Culture Techniques
Diabetes Mellitus, Experimental - physiopathology
Diabetes Mellitus, Type 2 - physiopathology
Diabetic GK rat
Dose-Response Relationship, Drug
Fetal Blood - chemistry
In vitro assay for angiogenesis
Male
Neovascularization, Pathologic
Rats
Rats, Wistar
Streptozotocin-diabetic rat
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Summary:Angiogenesis of cultured choroids was quantitatively assayed in spontaneously diabetic GK and a bolus-treated streptozotocin (STZ)-diabetic Wistar rats. The number and total length of microvessels budded from cultured choroidal explants were measured to use as angiogenic indices. Both indices in 10-week-old Wistar rats were increased in parallel by 5% fetal bovine serum (FBS) from days 2 to 7 in culture. These indices in STZ-rats (10 weeks of age) were increased by 5% FBS to a greater extent than those in age-matched normal rats. These enhanced actions of FBS were concentration-dependent. The explants of 16-week-old GK rats also increased these indices to a greater extent than those of age-matched Wistar rats. Aging to 18 weeks of age also increased choroidal angiogenesis in the normal rats. In conclusion, the assay model of choroidal angiogenesis was established by determining the number and length of microvessels in cultured choroidal explants. The diabetic states of STZ-Wistar and GK rats enhanced FBS-induced choroidal angiogenesis. This assay model is useful for determining angiogenic activity of growth factors and effective drugs in diabetic choroidopathy and retinopathy.
ISSN:0021-5198
1347-3506
DOI:10.1254/jjp.78.471