Superiority of needle-free transdermal plasmid delivery for the induction of antigen-specific IFNgamma T cell responses in the dog

Although successful needle-free DNA vaccination has been described on several occasions, the true benefit of this delivery technology over needle-based injections for DNA vaccination of dogs has not yet been documented. We conducted a side-by-side comparison of needle-free transdermal plasmid delive...

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Bibliographic Details
Published in:Vaccine 2008-04, Vol.26 (18), p.2186-2190
Main Authors: Goubier, A, Fuhrmann, L, Forest, L, Cachet, N, Evrad-Blanchard, M, Juillard, V, Fischer, L
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Animals
Dogs
Injections, Intradermal
Injections, Intramuscular
Interferon-gamma - biosynthesis
Plasmids
T-Lymphocytes - immunology
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
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Summary:Although successful needle-free DNA vaccination has been described on several occasions, the true benefit of this delivery technology over needle-based injections for DNA vaccination of dogs has not yet been documented. We conducted a side-by-side comparison of needle-free transdermal plasmid delivery vs. intramuscular vs. intradermal needle-based delivery of the same plasmid in dogs. Our data confirmed the importance of the route of plasmid delivery and further established the unique potential of needle-free transdermal plasmid delivery to elicit strong antigen-specific, hTyr-specific IFNgamma T in the dog. Further, this study demonstrated that properly enabled DNA vaccination has the potential to trigger very significant cell-based immune responses in dogs, establishing needle-free transdermal plasmid delivery as a critical technology for successful immunotherapy of cancer and/or chronic infectious diseases in companion animal medicine.
ISSN:0264-410X
DOI:10.1016/j.vaccine.2008.01.059