Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor

We report here the generation of transgenic chickens that produce human granulocyte‐colony stimulating factor (hG‐CSF) using replication‐defective Moloney murine leukemia virus (MoMLV)‐based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV‐G). The recombinant retrovirus was injec...

Full description

Saved in:
Bibliographic Details
Published in:Molecular reproduction and development 2008-07, Vol.75 (7), p.1120-1126
Main Authors: Kwon, Mo Sun, Koo, Bon Chul, Choi, Bok Ryul, Park, Yoon-Yub, Lee, Young Man, Suh, Hun Suk, Park, Young Sik, Lee, Hoon Taek, Kim, Jin-Hoi, Roh, Ji Yeol, Kim, Nam-Hyung, Kim, Teoan
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Biological and medical sciences
Biotechnology
Cell Culture Techniques
Chick Embryo - physiology
Chickens
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
Genetic engineering
Genetic technics
Genetic Vectors
Granulocyte Colony-Stimulating Factor - biosynthesis
human granulocyte-colony stimulating factor (hG-CSF)
Humans
Male
Methods. Procedures. Technologies
Moloney murine leukemia virus
Ovum - cytology
Ovum - physiology
Plasmids
Retroviridae - genetics
retrovirus vector
Semen
Transgenic animals
Transgenic animals and transgenic plants
transgenic chicken
Vesicular stomatitis virus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report here the generation of transgenic chickens that produce human granulocyte‐colony stimulating factor (hG‐CSF) using replication‐defective Moloney murine leukemia virus (MoMLV)‐based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV‐G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector‐encoded hG‐GSF gene. The biological activity of the recombinant hG‐CSF was significantly higher than its commercially derived E. coli‐derived counterpart. Successful germline transmission of the transgene was also confirmed in G1 transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G1 progeny were dead within 1 month of hatching. Mol. Reprod. Dev. 75: 1120–1126, 2008. © 2008 Wiley‐Liss, Inc.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20860