Male Gamete Empowerment

The degree of spermatogenesis varies among infertile men from a barely compromised sperm number in the ejaculate to their complete absence—the condition known as azoospermia. The scarcity of gametes often represents a major hindrance to overcoming spermatogenic failure through the use of assisted fe...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2008-04, Vol.1127 (1), p.64-66
Main Authors: Takeuchi, Takumi, Neri, Queenie V., Palermo, Gianpiero D.
Format: Article
Language:English
Subjects:
Animals
Azoospermia - diagnosis
Azoospermia - therapy
Biopsy
Cell Culture Techniques - methods
Cell Differentiation
Embryonic Stem Cells - pathology
ESC
gametogenesis
Germ Cells - pathology
Humans
Male
male gamete empowerment
male genome cloning
Mice
neo-spermatogenesis
oogenesis
Ploidies
Reproductive Techniques, Assisted
Seminiferous Tubules - pathology
Spermatogenesis
Testis - pathology
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Summary:The degree of spermatogenesis varies among infertile men from a barely compromised sperm number in the ejaculate to their complete absence—the condition known as azoospermia. The scarcity of gametes often represents a major hindrance to overcoming spermatogenic failure through the use of assisted fertilization techniques. This induced us to attempt replication of the spermatozoon by “male genome cloning.” We then investigated whether it is possible to induce human spermatogenesis in host testes by transplanting germ cells obtained from human testicular biopsy specimens into mouse seminiferous tubules. After plating the sorted germ cells on a feeder layer, one putative spermatogonial stem cell colony proliferated up to day 9 of culture. The production of male gametes by ooplasmic somatic cell haploidization has been tested in our laboratory. Attempts to induce neogametogenesis from embryonic stem cells has yielded mouse offspring following fertilization with sperm‐like cells.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1434.016