A simple method of infecting rabbits with Bovine herpesvirus 1 and 5

This report describes an alternative technique to inoculate rabbits and to reproduce infection by Bovine herpesvirus 1 and 5. First, the nostrils are anaesthetized by aspersion with local anaesthetic. A few seconds later, and after proving the insensitivity of the zone, the rabbits are put on their...

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Bibliographic Details
Published in:Journal of virological methods 2008-06, Vol.150 (1), p.77-79
Main Authors: Valera, A.R., Pidone, C.L., Massone, A.R., Quiroga, M.A., Riganti, J.G., Corva, S.G., Galosi, C.M.
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Antibodies, Viral - blood
Biological and medical sciences
Bovine herpesvirus
Bovine herpesvirus 1
Disease Models, Animal
Encephalitis, Viral - physiopathology
Encephalitis, Viral - virology
Experimental infection
Female
Fundamental and applied biological sciences. Psychology
Herpesviridae Infections - physiopathology
Herpesviridae Infections - virology
Herpesvirus 1, Bovine - growth & development
Herpesvirus 1, Bovine - isolation & purification
Herpesvirus 5, Bovine - growth & development
Herpesvirus 5, Bovine - isolation & purification
Liver - pathology
Lung - pathology
Male
Meningoencephalitis - physiopathology
Meningoencephalitis - virology
Microbiology
Nose - virology
Rabbits
Techniques used in virology
Virology
Virology - methods
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Summary:This report describes an alternative technique to inoculate rabbits and to reproduce infection by Bovine herpesvirus 1 and 5. First, the nostrils are anaesthetized by aspersion with local anaesthetic. A few seconds later, and after proving the insensitivity of the zone, the rabbits are put on their back legs with their nostrils upwards and the inoculum is introduced slowly into each nostril by using disposable droppers. Clinical signs, viral isolation from nasal swabs, histological lesions found, positive polymerase chain reaction and antibodies production confirm the infection. This very simple and bloodless technique, where the animals are exposed to minor distress, may be useful for evaluating the virulence of BoHV-1 and BoHV-5 strains, to study the establishment of latent virus infection and to test the potential of experimental vaccines or properties of antiviral drugs. It may be also suitable for experimental infection with other respiratory viruses in this animal model.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2008.03.008