In vivo studies of the biosynthesis of vernolic acid in the seed of Vernonia galamensis

In vivo radiotracer experiments using [1‐14C]acetate as the precursor were conducted to investigate the biosynthesis of vernolic acid (12, 13‐epoxy‐cis‐9‐octadecenoic acid) in the seeds of Vernonia galamensis. The acetate precursor radioactively labeled vernolate in phosphatidylcholine (PC), diacylg...

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Bibliographic Details
Published in:Lipids 1998-12, Vol.33 (12), p.1217-1221
Main Authors: Liu, L. (Iowa State University, Ames, IA.), Hammond, E.G, Nikolau, B.J
Format: Article
Language:English
Subjects:
Acetates - metabolism
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Carbon Radioisotopes - metabolism
Epoxy Compounds - chemical synthesis
GRAINE
Lipid Metabolism
Oleic Acids - chemical synthesis
Plants - embryology
Plants - metabolism
Radioactive tracers
SEEDS
Seeds - metabolism
SEMILLA
VERNONIA
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Summary:In vivo radiotracer experiments using [1‐14C]acetate as the precursor were conducted to investigate the biosynthesis of vernolic acid (12, 13‐epoxy‐cis‐9‐octadecenoic acid) in the seeds of Vernonia galamensis. The acetate precursor radioactively labeled vernolate in phosphatidylcholine (PC), diacylglycerol, and triacylglycerol. Time‐course kinetics of the incorporation of the radioactive tracer indicated that vernolate is synthesized while the acyl moiety is esterified to PC. Pulsechase experiments provided additional supporting evidence that vernolate is synthesized while esterified to PC. These results are consistent with the hypothesis that linoleoyl PC is the precursor of vernoleoyl‐PC. Subsequently, vernolate is quickly moved from the PC pool to the triacylglycerol pool, where it accumulates.
ISSN:0024-4201
1558-9307
DOI:10.1007/s11745-998-0326-3