Successful isolation and long-term establishment of a cell line with stem cell-like features from an anaplastic medulloblastoma

Aims: Herein we report on the successful isolation and establishment of a novel, long‐term, primary, neurosphere‐like cell line called 1603‐MED from a 5‐year‐old boy affected by a highly aggressive anaplastic medulloblastoma. Methods: Elaboration of the new protocol for neurosphere assay is extensiv...

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Bibliographic Details
Published in:Neuropathology and applied neurobiology 2008-06, Vol.34 (3), p.306-315
Main Authors: Raso, A., Negri, F., Gregorio, A., Nozza, P., Mascelli, S., De Marco, P., Merello, E., Milanaccio, C., Ravegnani, M., Cama, A., Garrè, M. L., Capra, V.
Format: Article
Language:English
Subjects:
anaplasia
Biological and medical sciences
cancer stem cells
Capra
Cell Culture Techniques - methods
Cell Differentiation
Cell Line, Tumor - cytology
Cerebellar Neoplasms - pathology
Child, Preschool
Flow Cytometry
Hematologic and hematopoietic diseases
Humans
Immunohistochemistry
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
medulloblastoma
Medulloblastoma - pathology
Neurology
Neurons - cytology
paediatric brain tumours
Stem Cells - cytology
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Summary:Aims: Herein we report on the successful isolation and establishment of a novel, long‐term, primary, neurosphere‐like cell line called 1603‐MED from a 5‐year‐old boy affected by a highly aggressive anaplastic medulloblastoma. Methods: Elaboration of the new protocol for neurosphere assay is extensively discussed, together with a complete immuno‐histochemical and cytogenetic characterization of 1603‐MED. Results: Clinical course and histopathology are briefly discussed. The 1603‐MED possesses a high capacity for proliferation, CD133 expression, self‐renewal and differentiation, thus indicating that anaplastic medulloblastoma contains a subpopulation of cancer stem cells as observed in classic medulloblastoma. Conclusions: 1603‐MED provides us with the first in vitro model of anaplastic medulloblastoma that may be suitable for studying both tumour progression and the genetic mechanisms related to therapy resistance, and may lead to the development and testing of chemosensitivity and new therapeutic targets.
ISSN:0305-1846
1365-2990
DOI:10.1111/j.1365-2990.2007.00896.x