nucleotide sequence of the 3'-terminal region of dasheen mosaic virus (Caladium isolate) and expression of its coat protein in Escherichia coli for antiserum production

• Published in: Archives of virology 1998-01, Vol.143 (12), p.2461-2469
• Main Authors: Li, R.H, Zettler, F.W, Purcifull, D.E, Hiebert, E
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Amino acids; Animals; Antibodies, Viral - biosynthesis; antigens; Antisera; antiserum; Base Sequence; Bean common mosaic virus; Biological and medical sciences; Caladium; Capsid - genetics; Capsid - immunology; Coat protein; coat proteins; complementary DNA; Dasheen mosaic virus; DNA Primers - genetics; DNA, Complementary - genetics; Enzyme-linked immunosorbent assay; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; genbank/af048981; Gene Expression; genes; Genetics; Microbiology; Molecular Sequence Data; Nucleotide sequence; nucleotide sequences; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant viruses; Plant viruses and viroids; Plants - virology; Potyvirus - genetics; Potyvirus - immunology; Potyvirus - isolation & purification; Rabbits; Recombinant Proteins - genetics; Recombinant Proteins - immunology; RNA; RNA, Viral - genetics; Sequence Homology, Amino Acid; Systematics. Structure, properties and multiplication. Genetics; taro; Virology; Western blotting
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s007050050476

A caladium isolate of dasheen mosaic virus (DsMV-Ch) was cloned as cDNA from genomic RNA. The sequence of the 3′-terminal 3 158 nucleotides, which consisted of the 3′-terminus of the NIa gene, the NIb gene, the coat protein (CP) gene, and a 246-nucleotide non-coding region, was between 57–68% similar at the nucleotide level and 72–82% similar at the amino acid level when compared with other potyviruses. Phylogenetic analysis of aligned, selected potyviral CP sequences indicate that DsMV-Ch is similar to DsMV isolates infecting taro and closely related to the bean common mosaic virus subgroup in the genus Potyvirus. A recombinant DsMV-Ch CP (∼39 kDa) expressed in E. coli was used as an immunogen and the resulting antiserum reacted with DsMV and several other potyviruses in Western blots and indirect ELISA.