Immunohistochemical localization of serotonin transporter in normal and colchicine treated rat brain

Distribution of serotonin transporter (SET) was examined immunohistochemically in the rat brain using two specific polyclonal antibodies raised against oligopeptides corresponding with 15 amino acids of carboxyl terminus and 14 amino acids of amino terminus of rat SET. The distribution and density o...

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Bibliographic Details
Published in:Neuroscience research 1998-12, Vol.32 (4), p.305-312
Main Authors: Yamamoto, Hiroshi, Fujimiya, Mineko, Shirai, Yasuhito, Nakashita, Mayumi, Oyasu, Miho, Saito, Naoaki
Format: Article
Language:English
Subjects:
Animals
Brain Chemistry
Carrier Proteins - analysis
Cerebral Cortex - chemistry
Cerebral Cortex - drug effects
Colchicine
Colchicine - pharmacology
Female
Immunohistochemistry
Male
Membrane Glycoproteins - analysis
Membrane Transport Proteins
Nerve Tissue Proteins
Rabbits
Raphe nuclei
Raphe Nuclei - chemistry
Raphe Nuclei - drug effects
Rat
Rats
Rats, Wistar
Serotonin
Serotonin Plasma Membrane Transport Proteins
Serotonin transporter
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Summary:Distribution of serotonin transporter (SET) was examined immunohistochemically in the rat brain using two specific polyclonal antibodies raised against oligopeptides corresponding with 15 amino acids of carboxyl terminus and 14 amino acids of amino terminus of rat SET. The distribution and density of SET immunoreactive varicose fibers were quite similar to those of serotonin immunoreactive fibers, however no neuronal cell bodies in the brainstem raphe nuclei was stained in normal rat brain. Electron microscopic study showed that SET immunoreactivity was predominantly localized in the presynaptic terminals. After intraventricular infusion of colchicine, neuronal perikarya of dorsal, median, and pontine raphe nuclei became visible. These results suggest that SET is likely present at the synaptic terminals of serotonergic neurons and such localization may be in good agreement with its pharmacological action which includes reuptake of serotonin at presynaptic nerve terminals.
ISSN:0168-0102
1872-8111
DOI:10.1016/S0168-0102(98)00097-2