Up-regulation of the cellular level of Escherichia coli PTS components by stabilizing reduced transcripts of the genes in response to the low oxygen level

When Escherichia coli cells were grown with limited levels of oxygen, the glucose-induced transcription of ptsG was decreased whereas deletion of the arcA gene partially restored it, which was consistent with the previous report that the ArcA protein represses ptsG transcription. However, under this...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-06, Vol.370 (4), p.609-612
Main Authors: Shin, Dongwoo, Cho, Namwook, Kim, You-Jin, Seok, Yeong-Jae, Ryu, Sangryeol
Format: Article
Language:English
Subjects:
Anaerobiosis - genetics
ArcA
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Expression Regulation, Bacterial
Glucose - metabolism
Glucose - pharmacology
mRNA stability
Operon - genetics
Oxygen - metabolism
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism
pts
RNA Stability
RNA, Bacterial - metabolism
RNA, Messenger - metabolism
Transcription, Genetic
Up-Regulation
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Summary:When Escherichia coli cells were grown with limited levels of oxygen, the glucose-induced transcription of ptsG was decreased whereas deletion of the arcA gene partially restored it, which was consistent with the previous report that the ArcA protein represses ptsG transcription. However, under this circumstance, we found that the level of EIICB Glc protein encoded by the ptsG gene was rather increased. This paradoxical phenomenon can be explained by the delayed turnover of ptsG mRNA in cells anaerobically grown in the presence of glucose. Finally, our data showed that anaerobic expression of the ptsHIcrr operon is also enhanced by increasing the longevity of the reduced mRNA levels.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.03.145