Evaluation of Bone Marrow Mesenchymal Stem Cell Standard Cryopreservation Procedure Efficiency

Abstract Introduction Mesenchymal stem cells are obtained from a variety of sources, particularly bone marrow. These cells have great potential for clinical research due to their potential to regenerate tissue. As is well known, the cryopreservation process can store any cell type, particularly bloo...

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Bibliographic Details
Published in:Transplantation proceedings 2008-04, Vol.40 (3), p.839-841
Main Authors: Carvalho, K.A.T, Cury, C.C, Oliveira, L, Cattaned, R.I.I, Malvezzi, M, Francisco, J.C, Pachalok, A, Olandoski, M, Faria-Neto, J.R, Guarita-Souza, L.C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Marrow Cells - cytology
Cell Adhesion
Cell Culture Techniques
Cell Differentiation - physiology
Cell Separation - methods
Cell Survival
Cryopreservation - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Male
Medical sciences
Mesenchymal Stromal Cells - cytology
Rats
Rats, Wistar
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
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Summary:Abstract Introduction Mesenchymal stem cells are obtained from a variety of sources, particularly bone marrow. These cells have great potential for clinical research due to their potential to regenerate tissue. As is well known, the cryopreservation process can store any cell type, particularly blood cells, for an indeterminate time. Objective The aim of this study was to analyze the efficiency of standard cryopreservation procedures for adult mesenchymal stem cells from bone marrow. Methods Mononuclear stem cells isolated from 10 Wistar male rats were cultivated for 4 weeks to obtain mesenchymal stem cells. The parameters considered in this study were trypan blue exclusion test and annexin V conjugated with 7-amino-actinomycin for flow cytometry before cryopreservation in liquid nitrogen vapor phase for 1 month and after thawing. Results The viabilities determined by the trypan blue exclusion test were 94.76% and 90.58%, and the flow cytometry assay (annexin V conjugated with 7-amino-actinomycin) were 85.52% and 66.25%, before cryopreservation and after thawing, respectively. Conclusions Standard procedures for cryopreservation were not efficient for those cells. The flow cytometry assay was more sensitive than the trypan blue exclusion test to demonstrate nonviability.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2008.03.004