MMP-9 protein level does not reflect overall MMP activity in the airways of patients with COPD

Summary Background Animal and human studies have implicated an imbalance of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in the pathogenesis of chronic obstructive pulmonary disease (COPD). MMP-9 protein is increased in COPD and we hypothesized that tot...

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Bibliographic Details
Published in:Respiratory medicine 2008-06, Vol.102 (6), p.845-851
Main Authors: Lowrey, Gillian E, Henderson, Neil, Blakey, John D, Corne, Jonathan M, Johnson, Simon R
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomarkers - metabolism
Bronchoalveolar Lavage Fluid - chemistry
Chronic obstructive pulmonary disease
Chronic obstructive pulmonary disease, asthma
Collagenases - metabolism
Female
Forced Expiratory Volume
Gelatinases - metabolism
Humans
Induced sputum
Male
Matrix metalloproteinase
Matrix Metalloproteinase 9 - metabolism
Matrix Metalloproteinases - metabolism
Medical sciences
Middle Aged
Pancreatic Elastase - metabolism
Pneumology
Proteins
Pulmonary Disease, Chronic Obstructive - enzymology
Pulmonary Disease, Chronic Obstructive - metabolism
Pulmonary Disease, Chronic Obstructive - physiopathology
Pulmonary/Respiratory
Sputum - enzymology
Sputum - metabolism
Studies
Thoracic surgery
Tissue Inhibitor of Metalloproteinase-1 - metabolism
Tissue Inhibitor of Metalloproteinase-2 - metabolism
Tissue inhibitors of matrix metalloproteinases
Vital Capacity
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Summary:Summary Background Animal and human studies have implicated an imbalance of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in the pathogenesis of chronic obstructive pulmonary disease (COPD). MMP-9 protein is increased in COPD and we hypothesized that total MMP activity would be raised although this has not previously been measured. Methods Using fluorescence and biotin labelled MMP assays, RT-PCR, western blotting and enzyme-linked immunosorbent assay we examined total MMP activity, specific gelatinase, elastase, collagenase activity, TIMP-1 and TIMP-2 in induced sputum from smokers with COPD and smokers without COPD. Results Induced sputum was obtained from 15 smokers with COPD and 14 smokers without COPD. MMP-9 levels were higher in those with COPD compared with controls ( p
ISSN:0954-6111
1532-3064
DOI:10.1016/j.rmed.2008.01.006