Phenolic Composition in Grape (Vitis vinifera L. cv. Malbec) Ripened with Different Solar UV-B Radiation Levels by Capillary Zone Electrophoresis

The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (−UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluat...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-05, Vol.56 (9), p.2892-2898
Main Authors: Berli, Federico, D’Angelo, José, Cavagnaro, Bruno, Bottini, Rubén, Wuilloud, Rodolfo, Silva, M. Fernanda
Format: Article
Language:English
Subjects:
accuracy
Analytical Methods
Anthocyanins - analysis
Biological and medical sciences
capillary electrophoresis
capillary zone electrophoresis
Electrophoresis, Capillary
Flavonoids - analysis
food analysis
food composition
Food industries
Fruit - chemistry
Fruit - growth & development
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
geographical variation
grapevines
pericarp
Phenolic composition
phenolic compounds
Phenols - analysis
Polyphenols
rapid methods
ripening
solar radiation
Stilbenes - analysis
Sunlight
ultraviolet radiation
Ultraviolet Rays
UV-B radiation
Vitis - chemistry
Vitis - growth & development
Vitis vinifera
Vitis viniferaL. cv. Malbec
wine grapes
wines
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Summary:The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (−UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the −UV-B treatment at this altitude.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf073421+