Role of metalloproteinases at the onset of liver development

At the onset of liver development, the hepatic precursor cells, namely, the hepatoblasts, derive from the ventral foregut endoderm and form a bud surrounded by a basement membrane (BM). To initiate liver growth, the hepatoblasts migrate across the BM and invade the neighboring septum transversum mes...

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Bibliographic Details
Published in:Development, growth & differentiation growth & differentiation, 2008-06, Vol.50 (5), p.331-338
Main Authors: Margagliotti, Sabrina, Clotman, Frédéric, Pierreux, Christophe E, Lemoine, Pascale, Rousseau, Guy G, Henriet, Patrick, Lemaigre, Frédéric P
Format: Article
Language:English
Subjects:
Animals
Basement Membrane - embryology
Cell Differentiation - physiology
Cell Movement - physiology
hepatoblast migration
Hepatocytes - cytology
Hepatocytes - enzymology
Liver - cytology
Liver - embryology
Liver - enzymology
liver development
Matrix Metalloproteinase 14 - physiology
Matrix Metalloproteinase 2 - physiology
matrix metalloproteinases
Matrix Metalloproteinases - physiology
Mice
Mice, Inbred Strains
Organogenesis - physiology
Stem Cells - cytology
Stem Cells - enzymology
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Summary:At the onset of liver development, the hepatic precursor cells, namely, the hepatoblasts, derive from the ventral foregut endoderm and form a bud surrounded by a basement membrane (BM). To initiate liver growth, the hepatoblasts migrate across the BM and invade the neighboring septum transversum mesenchyme. In the present study, carried out in the mouse embryo, we searched for effectors involved in this process and we examined the role of matrix metalloproteinases (MMPs). We found expression of a broad range of MMPs, among which MMP-2 was predominantly expressed in the septum transversum and MMP-14 in the hepatoblasts. Using a new liver explant culture system we showed that inhibition of MMP activity represses migration of the hepatoblasts. We conclude that MMPs are required to initiate expansion of the liver during development and that our culture system provides a new model to study hepatoblast migration.
ISSN:0012-1592
1440-169X
DOI:10.1111/j.1440-169X.2008.01031.x