Extraction of equine chorionic gonadotrophin from pregnant mare plasma by direct adsorption on chromatographic media

• Published in: Biotechnology and bioengineering 1998-01, Vol.57 (1), p.22-25
• Main Authors: González, Gualberto, Castro, Beatriz, Massaldi, Hugo
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; batch sorption; Biological and medical sciences; Biotechnology; Chromatography, Ion Exchange; Column chromatography; eCG; Extraction; Female; Fundamental and applied biological sciences. Psychology; Gonadotropins, Equine - blood; Gonadotropins, Equine - isolation & purification; hormone purification; Horses; Ion Exchange Resins; Ionic strength; Living systems studies; Methods. Procedures. Technologies; Others; Plasma applications; Pregnancy; pregnant mare serum gonadotrophin; Purification; Solvents; Various methods and equipments
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(19980105)57:1<22::AID-BIT3>3.0.CO;2-X

Equine chorionic gonadotrophin (eCG) is a hormone of practical value in veterinary medicine and animal production. Here we report a novel preparation procedure based on its direct adsorption onto anionic‐exchange resins in a batch‐wise mode. The active plasma is previously conditioned to reduce pH and ionic strength to required levels. After the adsorption stage, a 90% recovery of the initial eCG is achieved, with a concentration factor of about 50 and an enrichment factor around 500, with high preservation of biological activity. Further purification is carried out by cation‐exchange column chromatography. The recovery for the whole process is higher than 70%, and the final potency of the preparation is close to 4000 IU/mg. The process is well suited for its application to the industrial scale. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 22–25, 1998.